Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Dnase2btm1Osa
Name:	deoxyribonuclease II beta; targeted mutation 1, Shigekazu Nagata
MGI ID:	MGI:2675502
Synonyms:	DLAD-
Gene:	Dnase2b  Location: Chr3:146286740-146321351 bp, - strand  Genetic Position: Chr3, 72.0 cM
Alliance:	Dnase2btm1Osa page

Germline Transmission:	Earliest citation of germline transmission: J:85215
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	Not Specified

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: Exons 3 and 4 were replaced with a neomycin selection cassette inserted by homologous recombination. Included in the deleted region were codons for 2 conserved histidine residues that are putative enzymatic active sites. Truncated transcript lacking the targeted sequence, and consequently containing a frameshift mutation, was detected in mutant mice by both Northern and RT-PCR analyses. Absence of DNAse activity under acidic conditions indicated that stable and functional protein was not produced by the mutant transcript. (J:85215)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dnase2b Mutation:	23 strains or lines available

Original:	J:85215 Nishimoto S, et al., Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens. Nature. 2003 Aug 28;424(6952):1071-4
All:	2 reference(s)