Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Mt1tm1Rch
Name:	metallothionein 1; targeted mutation 1, Anna E Michalska
MGI ID:	MGI:2668454
Synonyms:	MT-I -, MT-null
Gene:	Mt1  Location: Chr8:94905710-94906955 bp, + strand  Genetic Position: Chr8, 46.34 cM
Alliance:	Mt1tm1Rch page

Germline Transmission:	Earliest citation of germline transmission: J:14416
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: Both metallothionein I and II were disrupted using the same targeting construct. Exon 1 of metallothionein I was disrupted by insertion of a 20 bp synthetic oligomer just downstream from the start codon which caused a frame shift. Northern blot analysis of liver mRNA from homozygotes in which the gene was induced by intraperitoneal injection of copper indicated that transcription did take place in spite of the frame shift. However, translation of the protein product did not occur. (J:14416)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Mt1 Mutation:	49 strains or lines available

Original:	J:14416 Michalska AE, et al., Targeting and germ-line transmission of a null mutation at the metallothionein I and II loci in mouse. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8088-92
All:	26 reference(s)