Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Cd28tm1Pjl
Name:	CD28 antigen; targeted mutation 1, Philip J Lucas
MGI ID:	MGI:2664380
Synonyms:	CD28-
Gene:	Cd28  Location: Chr1:60785547-60812521 bp, + strand  Genetic Position: Chr1, 30.52 cM, cytoband C1-C3
Alliance:	Cd28tm1Pjl page

Germline Transmission:	Earliest citation of germline transmission: J:25536
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The gene was disrupted by replacement of a 0.5 kb region containing most of exon 2 with a neomycin resistance cassette. Exon 2 encodes the Ig-like region, an alternative splice site, a putative ligand-binding domain, and represents more than 70% of the extracellular domain. Absence of gene expression was verified by flow cytometry of CD4+ T cells from homozygous mutant animals. (J:25536)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cd28 Mutation:	53 strains or lines available

Original:	J:25536 Lucas PJ, et al., Naive CD28-deficient T cells can initiate but not sustain an in vitro antigen-specific immune response. J Immunol. 1995 Jun 1;154(11):5757-68
All:	5 reference(s)