Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Nos3tm1Weo
Name:	nitric oxide synthase 3, endothelial cell; targeted mutation 1, William E O'Brien
MGI ID:	MGI:2657240
Synonyms:	eNOS-, Nos3m
Gene:	Nos3  Location: Chr5:24569808-24589472 bp, + strand  Genetic Position: Chr5, 11.32 cM
Alliance:	Nos3tm1Weo page

Germline Transmission:	Earliest citation of germline transmission: J:51563
Parent Cell Line:	AB2.1 (ES Cell)
Strain of Origin:	129S7/SvEvBrd-Hprt1b-m2

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The gene was disrupted by replacement of exon 1 with a neomycin resistance cassette via homologous recombination. The gene targeting event results in deletion of the first 54 amino acids and a frameshift of the remaining sequence. Absence of gene expression in homozygous mutant animals was confirmed by immunohistochemistry. (J:51563)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nos3 Mutation:	56 strains or lines available

Original:	J:51563 Gregg AR, et al., Limb reduction defects in endothelial nitric oxide synthase-deficient mice. Am J Physiol. 1998 Dec;275(6 Pt 2):H2319-24
All:	7 reference(s)