Car9tm1Spas
Targeted Allele Detail
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Symbol: |
Car9tm1Spas |
Name: |
carbonic anhydrase 9; targeted mutation 1, Silvia Pastorekova |
MGI ID: |
MGI:2448129 |
Synonyms: |
CA IX KO, Car9- |
Gene: |
Car9 Location: Chr4:43507026-43513729 bp, + strand Genetic Position: Chr4, 23.05 cM
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Alliance: |
Car9tm1Spas page
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Morphological changes in the stomach and intestine of Car2n/Car2n and Car9tm1Spas/Car9tm1Spas mice
Show the 1 phenotype image(s) involving this allele.
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Germline Transmission: |
Earliest citation of germline transmission:
J:80329
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Parent Cell Line: |
E14 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A PGK-neo cassette was inserted into exon 1 by homologous recombination. The insertion of neo downstream of the signal peptide deleted 14 nt (268-282) of the proteoglycan domain and caused a frameshift mutation. RT-PCR analysis of homozygous mutant mice identified a frame shifted transcript lacking 5' sequence encoding the signal peptide and most of the proteoglycan domain. Though the mutant transcript contained 77 nt of the neo cassette, a 3' portion of exon 1, and all of exons 2 through 11, protein was undetected by Western blot analysis of stomach tissue.
(J:80329)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:80329 Gut MO, et al., Gastric hyperplasia in mice with targeted disruption of the carbonic anhydrase gene Car9. Gastroenterology. 2002 Dec;123(6):1889-903 |
All: |
10 reference(s) |
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