Tcrgtm1Iku
Targeted Allele Detail
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| Symbol: |
Tcrgtm1Iku |
| Name: |
T cell receptor gamma chain; targeted mutation 1, Koichi Ikuta |
| MGI ID: |
MGI:2429947 |
| Synonyms: |
TCR-Vgamma4/6 -, TCR Vgamma4/6 KO, Vgammadelta |
| Gene: |
Tcrg Location: Chr13:19362212-19540646 bp, + strand Genetic Position: Chr13, cytoband A2-A3
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| Germline Transmission: |
Earliest citation of germline transmission:
J:80156
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| Parent Cell Line: |
E14.1 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutation: |
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Intragenic deletion
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Mutation details: A targeting vector containing a Tcrg-V2 gene modified to lack a restriction site, a floxed Tcrg-V4 gene, and a Tcrg-V3 gene also modified to lack a restriction site was electroporated into ES cells. The ES cells were then transfected with cre recombinase, resulting in the excision of Tcrg-V4. Tcrg-V2 was left adjacent to Tcrg-V3 (with single loxP site in between). Southern blot analysis indicated that only a portion of the targeting vector recombined as the modified Tcrg-V2 and the endogenous Tcrg-V3 (not the modified Tcrg-V3) were present.
(J:39791)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Tcrg Mutation: |
1 strain or line available
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| Original: |
J:80156 Sunaga S, et al., Efficient removal of loxP-flanked DNA sequences in a gene-targeted locus by transient expression of Cre recombinase in fertilized eggs. Mol Reprod Dev. 1997 Feb;46(2):109-13 |
| All: |
14 reference(s) |
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Analysis Tools
