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Camk4tm2Arm
Targeted Allele Detail
Summary
Symbol: Camk4tm2Arm
Name: calcium/calmodulin-dependent protein kinase IV; targeted mutation 2, Anthony R Means
MGI ID: MGI:2388343
Synonyms: CaSKO
Gene: Camk4  Location: Chr18:33067984-33324281 bp, + strand  Genetic Position: Chr18, 18.15 cM
Alliance: Camk4tm2Arm page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:70856
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129/Sv
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsThe calspermin proximal promoter and testis-specific exon were replaced with a floxed TK-neo cassette via homologous recombination. Transient expression of cre recombinase in correctly targeted ES cells removed the selection cassette leaving behind a single loxP site. The gene targeting strategy is designed to specifically delete calspermin expression but not Camk4 kinase expression. Northern blot of testis RNA confirmed the absence of calspermin transcript. Northern blot of testis and thymus RNA showed that Camk4 kinase expression was unaffected. (J:70856)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Camk4 Mutation:  37 strains or lines available
References
Original:  J:70856 Wu JY, et al., Spermatogenesis and the regulation of Ca(2+)-calmodulin-dependent protein kinase IV localization are not dependent on calspermin. Mol Cell Biol. 2001 Sep;21(17):6066-70
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory