Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | Notes | References

Symbol:	Fli1tm1Dkw
Name:	Friend leukemia integration 1; targeted mutation 1, Dennis K Watson
MGI ID:	MGI:2386167
Gene:	Fli1  Location: Chr9:32333500-32454157 bp, - strand  Genetic Position: Chr9, 17.74 cM
Alliance:	Fli1tm1Dkw page

Germline Transmission:	Earliest citation of germline transmission: J:63242
Parent Cell Line:	TC1/TC-1 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Hypomorph) Mutation:    Insertion   Mutation details: A floxed neo cassette was inserted into exon 9 via homologous recombination resulting in a premature stop codon between the DNA-binding domain and the CTA domain. The targeted mutation results in a hypomorphic allele. Northern blot analysis of homozygous mutant embryos showed 2 gene transcripts at 10% the level of wild-type transcription. Western blot analysis detected a truncated protein expressed at very low levels. Enzyme assay determined that the truncated protein exhibits 50%-60% of the activity of normal protein. (J:63242)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Fli1 Mutation:	26 strains or lines available

Original:	J:63242 Spyropoulos DD, et al., Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor. Mol Cell Biol. 2000 Aug;20(15):5643-52
All:	18 reference(s)