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Flt3tm1Irl
Targeted Allele Detail
Summary
Symbol: Flt3tm1Irl
Name: FMS-like tyrosine kinase 3; targeted mutation 1, Ihor R Lemischka
MGI ID: MGI:2384051
Synonyms: Flk2-
Gene: Flt3  Location: Chr5:147267551-147337299 bp, - strand  Genetic Position: Chr5, 86.88 cM, cytoband G3
Alliance: Flt3tm1Irl page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:27238
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsExon 3 was disrupted by the insertion of a neomycin selection cassette at the codon corresponding to residue 59 in the mature protein. An RNAse protection assay detected low levels of transcript in homozygous mutant mice. RT-PCR and sequence analyses of the mutant transcript showed that the targeted exon 3 had been excised, resulting in a frameshift mutation that introduces a stop codon after codon 51. (J:27238)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 12 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Flt3 Mutation:  83 strains or lines available
References
Original:  J:27238 Mackarehtschian K, et al., Targeted disruption of the flk2/flt3 gene leads to deficiencies in primitive hematopoietic progenitors. Immunity. 1995 Jul;3(1):147-61
All:  42 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory