Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Grk5tm1Rjl
Name:	G protein-coupled receptor kinase 5; targeted mutation 1, Robert J Lefkowitz
MGI ID:	MGI:2383968
Synonyms:	grk5-, GRK5 KO, GRK5-KO
Gene:	Grk5  Location: Chr19:60878187-61084406 bp, + strand  Genetic Position: Chr19, 56.52 cM
Alliance:	Grk5tm1Rjl page

Germline Transmission:	Earliest citation of germline transmission: J:59179
Parent Cell Line:	AK7 (ES Cell)
Strain of Origin:	129S4/SvJaeSor

Allele Type:    Targeted (Null/knockout) Mutation:    Intragenic deletion   Mutation details: A loxP site was inserted upstream of exon 7 and a floxed TK-neo derived cassette was inserted downstream of exon 8 via homologous recombination. Exons 7 and 8 encode critical subelements I, II, and III of the protein kinase catalytical domain. The floxed region containing exons 7 and 8 and the TK-neo cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Western blot analysis of brain membranes from homozygous mutant animals confirmed the absence of gene expression. (J:59179)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

5 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Grk5 Mutation:	43 strains or lines available

Original:	J:59179 Gainetdinov RR, et al., Muscarinic supersensitivity and impaired receptor desensitization in G protein-coupled receptor kinase 5-deficient mice. Neuron. 1999 Dec;24(4):1029-36
All:	22 reference(s)