Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Kif3ctm1Gsn
Name:	kinesin family member 3C; targeted mutation 1, Lawrence S B Goldstein
MGI ID:	MGI:2183787
Synonyms:	Kif3ctypeI
Gene:	Kif3c  Location: Chr12:3415132-3456494 bp, + strand  Genetic Position: Chr12, 1.77 cM
Alliance:	Kif3ctm1Gsn page

Germline Transmission:	Earliest citation of germline transmission: J:70607
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutation:    Intragenic deletion   Mutation details: Two loxP sites were introduced to the intron and 5' untranslated region flanking exon 1, and a third loxP site was introduced with a PGK-TK/PGK-neo cassette directly downstream exon 1 via homologous recombination. Exon 1 and the PGK-TK/PGK-neo cassette were removed by transient expression of cre recombinase in correctly targeted ES cells resulting in the deletion of 2.1 kb of DNA encoding amino acids 1-518 which include the whole motor region and half of the alpha-helical coiled coil domains. Northern blot analysis detected a truncated transcript encoding the tail end of the gene in homozygous mutant animals. Truncated protein product was detected in homozygous mutants by Western blot analysis using antibodies recognizing the tail end of the protein. Immunoprecipitation of brain lysates from homozygous mutants demonstrated the ability of the truncated protein to specifically bind Kif3a and not Kif3b. (J:70607)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Kif3c Mutation:	24 strains or lines available

Original:	J:70607 Yang Z, et al., Functional analysis of mouse kinesin motor kif3c. Mol Cell Biol. 2001 Aug;21(16):5306-11
All:	1 reference(s)