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Del(8Pcid2-Cul4a)1Ktc
Targeted Allele Detail
Summary
Symbol: Del(8Pcid2-Cul4a)1Ktc
Name: deletion, Chr 8, Kristin T Chun 1
MGI ID: MGI:2183544
Synonyms: CUL-4A-delta1, Cul4a/Pcid2tm1Ktc
Gene: Del(8Pcid2-Cul4a)1Ktc  Location: unknown  Genetic Position: Chr8, Syntenic
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:78032
Parent Cell Line:  CCE916 (ES Cell)
Strain of Origin:  129S/SvEv
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intergenic deletion, Intragenic deletion
  Del(8Pcid2-Cul4a)1Ktc involves 2 genes/genome features (Pcid2, Cul4a) View all
 
Mutation detailsA floxed region containing exon 1 and approximately 1.1 kb of upstream sequence was deleted by the expression of cre recombinase in vitro. Though sequence analysis showed the deletion to generate an in frame translational start codon at position 146, a truncated protein product was undetected by Western blot analysis of homozygous mutant mice. In a subsequent publication (J:150427), the authors demonstrate that that the upstream loxP site was inserted in a separate gene, Pcid2, that lies in a head-to-head configuration with Cul4a. This loxP site, inserted in a NotI restriction site, lies downstream of the putative translational start site of the gene. Thus the cre-mediated excision potentially disrupted both genes. RNAi knockdown of the Pcid2 transcript in ES cells suggests the embryonic lethality associated with this allele is at least partially due to disruption of the Pcid2 gene. (J:78032, J:150427)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Del(8Pcid2-Cul4a)1Ktc Mutation:  0 strains or lines available
References
Original:  J:78032 Li B, et al., CUL-4A is critical for early embryonic development. Mol Cell Biol. 2002 Jul;22(14):4997-5005
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory