Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Rasgrf2tm1Esn
Name:	RAS protein-specific guanine nucleotide-releasing factor 2; targeted mutation 1, Eugenio Santos
MGI ID:	MGI:2183211
Synonyms:	grf2-, Rasgrf2-
Gene:	Rasgrf2  Location: Chr13:92028519-92268164 bp, - strand  Genetic Position: Chr13, 47.43 cM, cytoband C3-D1
Alliance:	Rasgrf2tm1Esn page

Germline Transmission:	Earliest citation of germline transmission: J:75928
Parent Cell Line:	CJ7 (ES Cell)
Strain of Origin:	129S1/Sv-Oca2+ Tyr+ Kitl+

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A portion of the CDC25-H domain, a highly conserved region of the gene, from amino acids 1005-1070 was replaced with a PGK-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by RT-PCR analysis of brain RNA and absence of protein product was demonstrated by Western blot of brain and lung extracts using an antibody directed against a region of the protein upstream of the disruption. (J:75928)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rasgrf2 Mutation:	73 strains or lines available

Original:	J:75928 Fernandez-Medarde A, et al., Targeted disruption of Ras-Grf2 shows its dispensability for mouse growth and development. Mol Cell Biol. 2002 Apr;22(8):2498-504
All:	8 reference(s)