Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Pgrtm1Omc
Name:	progesterone receptor; targeted mutation 1, Orla M Conneely
MGI ID:	MGI:2183208
Synonyms:	PRAKO-
Gene:	Pgr  Location: Chr9:8899834-8968612 bp, + strand  Genetic Position: Chr9, 2.46 cM
Alliance:	Pgrtm1Omc page

Germline Transmission:	Earliest citation of germline transmission: J:77661
Parent Cell Line:	AB2.2 (ES Cell)
Strain of Origin:	129S7/SvEvBrd-Hprt1b-m2

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Nucleotide substitutions   Mutation details: To eliminate translation of isoform A, without abrogating the translation of isoform B, the translational start site at codon 166, which is specific to isoform A, was modified to encode alanine. For selection, a silent mutation was introduced resulting in the generation of a novel NheI restriction site and a floxed neomycin selection cassette was inserted into intron 2. Recombinant ES cells were transfected with plasmids expressing cre recombinase, resulting in the excision of the neomycin cassette. Immunoblot analysis of uterine extracts from estrogen treated homozygous mutant mice showed an absence of isoform A. Isoform B protein levels were unaffected. (J:77661)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Pgr Mutation:	73 strains or lines available

Original:	J:77661 Mulac-Jericevic B, et al., Subgroup of reproductive functions of progesterone mediated by progesterone receptor-B isoform. Science. 2000 Sep 8;289(5485):1751-4
All:	6 reference(s)