Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol: Podxltm1Kmn Name: podocalyxin-like; targeted mutation 1, Kelly M McNagny MGI ID: MGI:2182833 Synonyms: podxl- Gene: Podxl  Location: Chr6:31496428-31540872 bp, - strand  Genetic Position: Chr6, 12.57 cM Alliance: Podxltm1Kmn page

Edema in Podxltm1Kmn/Podxltm1Kmn mice Show the 3 phenotype image(s) involving this allele.

Germline Transmission:	Earliest citation of germline transmission: J:70294
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The majority of exons 5-8 were replaced with a neomycin resistance gene in the opposite orientation via homologous recombination. The gene targeting event deletes 55 amino acids in the extracellular/juxtamembrane region, the transmembrane region, and the entire cytoplasmic tail of the encoded protein. Exons 5, 7, and 8 encode highly conserved domains across species. Absence of gene expression was demonstrated by Northern blot analysis of E16 fetal lung using a 5' region of the gene as a probe. Lack of gene expression was further verified by cell surface immunofluorescence analysis of E15 fetal liver cells using a monoclonal antibody to the mouse protein. (J:70294)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Podxl Mutation:	16 strains or lines available

Original:	J:70294 Doyonnas R, et al., Anuria, omphalocele, and perinatal lethality in mice lacking the CD34-related protein podocalyxin. J Exp Med. 2001 Jul 2;194(1):13-27
All:	5 reference(s)