Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Dio2tm1Vag
Name:	deiodinase, iodothyronine, type II; targeted mutation 1, Valerie Anne Galton
MGI ID:	MGI:2182450
Synonyms:	D2KO, Dio2-
Gene:	Dio2  Location: Chr12:90691326-90705812 bp, - strand  Genetic Position: Chr12, 44.05 cM
Alliance:	Dio2tm1Vag page

Germline Transmission:	Earliest citation of germline transmission: J:72958
Parent Cell Line:	AB2.2 (ES Cell)
Strain of Origin:	129S7/SvEvBrd-Hprt1b-m2

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A neomycin selection cassette replaced a 2.6kb region including a 5' portion of exon 2. 72% of the coding region was deleted, including a TGA codon that encodes the selenocysteine located at the active site. Northern blot analysis of total RNA obtained from brown adipose tissue showed an absence of transcript in homozygous mutant mice. Functional ablation of the endogenous protein in homozygous mutant mice was confirmed by assaying deiodination in brown adipose tissue. (J:72958)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	114 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dio2 Mutation:	18 strains or lines available

Original:	J:72958 Schneider MJ, et al., Targeted Disruption of the Type 2 Selenodeiodinase Gene (DIO2) Results in a Phenotype of Pituitary Resistance to T(4). Mol Endocrinol. 2001 Dec;15(12):2137-48
All:	40 reference(s)