Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Cenpbtm1Khac
Name:	centromere protein B; targeted mutation 1, K H Andy Choo
MGI ID:	MGI:2181521
Synonyms:	+/- neo, R1-
Gene:	Cenpb  Location: Chr2:131019209-131021974 bp, - strand  Genetic Position: Chr2, 63.29 cM
Alliance:	Cenpbtm1Khac page

Germline Transmission:	Earliest citation of germline transmission: J:47171
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: Stop codons were introduced into all three reading frames of the intronless gene by the insertion a translation frameshift linker into the amino-terminal centromere DNA-binding domain. An IRES-neomycin selection marker was inserted in the 3' untranslated region upstream of the poly adenylation signal. RT-PCR analysis of heterozygous mutant mice showed the expression of a truncated transcript. Mice possessing both this allele and Cenpbtm2Khac were shown to lack transcript and protein was not detected by immunofluorescence staining of metaphase chromosomes. (J:47171)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

6 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cenpb Mutation:	18 strains or lines available

Original:	J:47171 Hudson DF, et al., Centromere protein B null mice are mitotically and meiotically normal but have lower body and testis weights. J Cell Biol. 1998 Apr 20;141(2):309-19
All:	3 reference(s)