Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Lig1tm1Dwm
Name:	ligase I, DNA, ATP-dependent; targeted mutation 1, David W Melton
MGI ID:	MGI:2179227
Synonyms:	Lig1#53
Gene:	Lig1  Location: Chr7:13011239-13045350 bp, + strand  Genetic Position: Chr7, 7.82 cM
Alliance:	Lig1tm1Dwm page

Germline Transmission:	Earliest citation of germline transmission: J:76371
Parent Cell Line:	HM-1 (ES Cell)
Strain of Origin:	129P2/OlaHsd-Hprt1b-m3

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The 3' end of the gene (exons 23-27) was replaced with a PGK-HPRT minigene. Northern analysis failed to detect any intact, truncated, or fusion transcripts in homozygous mutant embryos. Western analysis failed to detect any protein in homozygous mutant embryos, when probed with antibodies against bovine DNA ligase 1. (J:76371)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Lig1 Mutation:	48 strains or lines available

Original:	J:76371 Bentley D, et al., DNA ligase I is required for fetal liver erythropoiesis but is not essential for mammalian cell viability. Nat Genet. 1996 Aug;13(4):489-91
All:	2 reference(s)