Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Il1btm1Dch
Name:	interleukin 1 beta; targeted mutation 1, David D Chaplin
MGI ID:	MGI:2157804
Synonyms:	IL-1beta-, IL-1BtmChaplin
Gene:	Il1b  Location: Chr2:129206490-129213059 bp, - strand  Genetic Position: Chr2, 62.97 cM, cytoband F
Alliance:	Il1btm1Dch page

Germline Transmission:	Earliest citation of germline transmission: J:33465
Parent Cell Line:	D3 (ES Cell)
Strain of Origin:	129S2/SvPas

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: A PGK-neomycin resistance cassette was inserted into exon 4 that resulted in an in-frame termination codon upstream of the ICE cleavage site. The authors predict that the receptor-binding domain of the protein would not be translated. Northern blot analysis detected no transcripts in LPS-stimulated peritoneal exudate cells from homozygous mice. Pulse-chase labeling and immunoprecipitation studies of thioglycollate-elicited PEC demonstrated that no detectable protein was present in homozygous mice. ELIZA analysis of ATP-treated PEC also did not detect protein produced by this allele. (J:33465)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Il1b Mutation:	24 strains or lines available

Original:	J:33465 Shornick LP, et al., Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone. J Exp Med. 1996 Apr 1;183(4):1427-36
All:	46 reference(s)