Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Ptprctm1Holm
Name:	protein tyrosine phosphatase receptor type C; targeted mutation 1, Nick Holmes
MGI ID:	MGI:1857277
Synonyms:	CD45-, CD45-exon9, Ptprctm1
Gene:	Ptprc  Location: Chr1:137990599-138103446 bp, - strand  Genetic Position: Chr1, 60.73 cM
Alliance:	Ptprctm1Holm page

Germline Transmission:	Earliest citation of germline transmission: J:33466
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: A neomycin resistance cassette, corrected for a point mutation believed to affect the transformation efficiency of ES cells, was used to disrupt exon 9, which is common to all of the isoforms. The mutation results in the introduction of several stop codons downstream of the insertion. FACS analysis using antibodies for multiple isoforms showed an absence of cell surface expression in homozygous mutant mice. A lack of encoded protein was further confirmed by Western blot analysis of thymus lysates. (J:33466)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 11 strains available      Cell Lines: 0 lines available
Carrying any Ptprc Mutation:	188 strains or lines available

Original:	J:33466 Byth KF, et al., CD45-null transgenic mice reveal a positive regulatory role for CD45 in early thymocyte development, in the selection of CD4+CD8+ thymocytes, and B cell maturation. J Exp Med. 1996 Apr 1;183(4):1707-18
All:	32 reference(s)