| Gene |
Genome Location (GRCm39) |
Reference |
QTL Note |
|
Cfl2 |
Chr12:54905594-54909662 (-) |
J:109430 |
It is believed that the enhanced diabetes susceptibility of C57BLKS/J-Lepr animals (compared to C57BL/6J-Lepr animals) is conferred by contribution of DBA/2J-derived DNA in the genome. It is estimated that 70% of the C57BLKS/J genome is of C57BL/6J origin, 20% is of DBA/2J origin, and 9% contributed by an unknown donor strain. Extensive SNP analysis using 15,300 SNPs at an average spacing of 167,000 bp on the autosomes and 330,000 bp on the X chromosome mapped blocks of C57BLKS/J genome derived from DBA/2J. In addition, expression analysis of genes in these blocks identified potential diabetes susceptibility candidate genes.
Previously identified QTL Ath6 at 3 cM on mouse Chromosome 12 mapped to one of these DBA/2J-derived blocks from 10.4 Mb to 19.5 Mb. Over 430 genes are found in this interval. Genes exhibiting significant expression differences between C57BL/6J and DBA/2J and correlating with diabetes-related traits were screened. Rrm2 (8 cM; 19.15 Mb; LOD=13.92), Dock4 (16.5 cM; 35.35 Mb; LOD=8.75), and Cfl2 (36.5 cM; 50.275 Mb; LOD=10.33) were identified as Ath6 candidate genes meeting the above criteria.
|
|
Dock4 |
Chr12:40495956-40896873 (+) |
J:109430 |
It is believed that the enhanced diabetes susceptibility of C57BLKS/J-Lepr animals (compared to C57BL/6J-Lepr animals) is conferred by contribution of DBA/2J-derived DNA in the genome. It is estimated that 70% of the C57BLKS/J genome is of C57BL/6J origin, 20% is of DBA/2J origin, and 9% contributed by an unknown donor strain. Extensive SNP analysis using 15,300 SNPs at an average spacing of 167,000 bp on the autosomes and 330,000 bp on the X chromosome mapped blocks of C57BLKS/J genome derived from DBA/2J. In addition, expression analysis of genes in these blocks identified potential diabetes susceptibility candidate genes.
Previously identified QTL Ath6 at 3 cM on mouse Chromosome 12 mapped to one of these DBA/2J-derived blocks from 10.4 Mb to 19.5 Mb. Over 430 genes are found in this interval. Genes exhibiting significant expression differences between C57BL/6J and DBA/2J and correlating with diabetes-related traits were screened. Rrm2 (8 cM; 19.15 Mb; LOD=13.92), Dock4 (16.5 cM; 35.35 Mb; LOD=8.75), and Cfl2 (36.5 cM; 50.275 Mb; LOD=10.33) were identified as Ath6 candidate genes meeting the above criteria.
|
|
Rrm2 |
Chr12:24758253-24764145 (+) |
J:109430 |
It is believed that the enhanced diabetes susceptibility of C57BLKS/J-Lepr animals (compared to C57BL/6J-Lepr animals) is conferred by contribution of DBA/2J-derived DNA in the genome. It is estimated that 70% of the C57BLKS/J genome is of C57BL/6J origin, 20% is of DBA/2J origin, and 9% contributed by an unknown donor strain. Extensive SNP analysis using 15,300 SNPs at an average spacing of 167,000 bp on the autosomes and 330,000 bp on the X chromosome mapped blocks of C57BLKS/J genome derived from DBA/2J. In addition, expression analysis of genes in these blocks identified potential diabetes susceptibility candidate genes.
Previously identified QTL Ath6 at 3 cM on mouse Chromosome 12 mapped to one of these DBA/2J-derived blocks from 10.4 Mb to 19.5 Mb. Over 430 genes are found in this interval. Genes exhibiting significant expression differences between C57BL/6J and DBA/2J and correlating with diabetes-related traits were screened. Rrm2 (8 cM; 19.15 Mb; LOD=13.92), Dock4 (16.5 cM; 35.35 Mb; LOD=8.75), and Cfl2 (36.5 cM; 50.275 Mb; LOD=10.33) were identified as Ath6 candidate genes meeting the above criteria.
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