Sema3a RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:270128 Schulz Y, et al., CHD7, the gene mutated in CHARGE syndrome, regulates genes involved in neural crest cell guidance. Hum Genet. 2014 Aug;133(8):997-1009
Assay type:	RT-PCR
MGI Accession ID:	MGI:7861762
Gene symbol:	Sema3a
Gene name:	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A

Probe:	Sema3a-pF, Sema3a-pR
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Tbp, Sdha, and Hprt. Wild-type values were set as 1.

Results

Image: 2_Sema3a

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
WT 1-4	E9.5	TS15: embryo	Present	5 µg; total RNA	involves: C3HeB/FeJ		Female
Het-1	E9.5	TS15: embryo	Present (a)	5 µg; total RNA	involves: C3HeB/FeJ	Chd7^Whi/Chd7⁺	Female
Het-2	E9.5	TS15: embryo	Present (b)	5 µg; total RNA	involves: C3HeB/FeJ	Chd7^Whi/Chd7⁺	Female
Het-3	E9.5	TS15: embryo	Present (c)	5 µg; total RNA	involves: C3HeB/FeJ	Chd7^Whi/Chd7⁺	Female
Het-4	E9.5	TS15: embryo	Present (d)	5 µg; total RNA	involves: C3HeB/FeJ	Chd7^Whi/Chd7⁺	Female
Homo-1	E9.5	TS15: embryo	Present (e)	5 µg; total RNA	involves: C3HeB/FeJ	Chd7^Whi/Chd7^Whi	Female
Homo-2	E9.5	TS15: embryo	Present (f)	5 µg; total RNA	involves: C3HeB/FeJ	Chd7^Whi/Chd7^Whi	Female
Homo-3	E9.5	TS15: embryo	Present (e)	5 µg; total RNA	involves: C3HeB/FeJ	Chd7^Whi/Chd7^Whi	Female
Homo-4	E9.5	TS15: embryo	Present (g)	5 µg; total RNA	involves: C3HeB/FeJ	Chd7^Whi/Chd7^Whi	Female

Notes:
(a) Expression was 60% of wild-type levels.
(b) Expression was 78% of wild-type levels.
(c) Expression was 52% of wild-type levels.
(d) Expression was 48% of wild-type levels.
(e) Expression was 38% of wild-type levels.
(f) Expression was 33% of wild-type levels.
(g) Expression was 31% of wild-type levels.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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