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Gene Expression Data
Assay Details
Assay
Reference: J:359190 Nagai M, et al., Neuronal splicing of the unmethylated histone H3K4 reader, PHF21A, prevents excessive synaptogenesis. J Biol Chem. 2024 Nov;300(11):107881
Assay type: RT-PCR
MGI Accession ID: MGI:7859301
Gene symbol: Phf21a
Gene name: PHD finger protein 21A
Probe: mPHF21A_F1, mPHF21A_R1
Probe preparation: labelled with SYBR green
Assay notes: Two primer sets were listed, but the primers for this assay were not specified. Either MGI:7858978 or MGI:7858979 could have been used.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
Embryonic days 12.5 E12.5 TS20: brain Present Present 1 µg; total RNA Not Specified Not Specified
Embryonic days 13.5 E13.5 TS21: brain Present Present 1 µg; total RNA Not Specified Not Specified
Embryonic days 14.5 E14.5 TS22: cerebral cortex Present Present 1 µg; total RNA Not Specified Not Specified
Embryonic days 15.5 E15.5 TS23: cerebral cortex Present Ambiguous 1 µg; total RNA Not Specified Not Specified
Embryonic days 16.5 E16.5 TS24: cerebral cortex Present Ambiguous 1 µg; total RNA Not Specified Not Specified
Embryonic days 17.5 E17.5 TS25: cerebral cortex Present Ambiguous 1 µg; total RNA Not Specified Not Specified
Embryonic days 18.5 E18.5 TS26: cerebral cortex Present Ambiguous 1 µg; total RNA Not Specified Not Specified
Embryonic days 19.5 E19.5 TS26: cerebral cortex Present Ambiguous 1 µg; total RNA Not Specified Not Specified
After birth (days) 0 P0 TS27: cerebral cortex Present Ambiguous 1 µg; total RNA Not Specified Not Specified
After birth (days) 1 P1 TS27: cerebral cortex Present Ambiguous 1 µg; total RNA Not Specified Not Specified
After birth (days) 2 P2 TS27: cerebral cortex Present Ambiguous 1 µg; total RNA Not Specified Not Specified
Notes:
(a) n isoform.
(b) c isoform. RT-PCR indicated that PHF21A mRNA largely switched from PHF21A-c to PHF21A-n after E15.5 mouse cortex.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory