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Gene Expression Data
Assay Details
Assay
Reference: J:359190 Nagai M, et al., Neuronal splicing of the unmethylated histone H3K4 reader, PHF21A, prevents excessive synaptogenesis. J Biol Chem. 2024 Nov;300(11):107881
Assay type: RT-PCR
MGI Accession ID: MGI:7859287
Gene symbol: Kdm1a
Gene name: lysine (K)-specific demethylase 1A
Probe: mLSD1_F1, mLSD1_R1
Probe preparation: labelled with SYBR green
Assay notes: Two primer sets were listed, but the primers for this assay were not specified. Either MGI:7858976 or MGI:7858974 could have been used.
Results
Image: 1D LSD1 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
Embryonic days 12.5 E12.5 TS20: brain Present Present 1 µg; total RNA Not Specified Not Specified
Embryonic days 13.5 E13.5 TS21: brain Present Present 1 µg; total RNA Not Specified Not Specified
Embryonic days 14.5 E14.5 TS22: cerebral cortex Present Present 1 µg; total RNA Not Specified Not Specified
Embryonic days 15.5 E15.5 TS23: cerebral cortex Present Present 1 µg; total RNA Not Specified Not Specified
Embryonic days 16.5 E16.5 TS24: cerebral cortex Present Present 1 µg; total RNA Not Specified Not Specified
Embryonic days 17.5 E17.5 TS25: cerebral cortex Present Present 1 µg; total RNA Not Specified Not Specified
Embryonic days 18.5 E18.5 TS26: cerebral cortex Present Present 1 µg; total RNA Not Specified Not Specified
Embryonic days 19.5 E19.5 TS26: cerebral cortex Present Present 1 µg; total RNA Not Specified Not Specified
After birth (days) 0 P0 TS27: cerebral cortex Present Present 1 µg; total RNA Not Specified Not Specified
After birth (days) 1 P1 TS27: cerebral cortex Present Present 1 µg; total RNA Not Specified Not Specified
After birth (days) 2 P2 TS27: cerebral cortex Present Present 1 µg; total RNA Not Specified Not Specified
Notes:
(a) n isoform. In the RT-PCR analysis, the LSD1-n/c ratio appeared to show stepwise increases on E16.5 and P0.
(b) c isoform. The LSD1-c was present throughout the developmental periods examined.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory