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Gene Expression Data
Assay Details
Assay
Reference: J:175804 Brzezinski JA 4th, et al., Ascl1 expression defines a subpopulation of lineage-restricted progenitors in the mammalian retina. Development. 2011 Aug;138(16):3519-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7855849
Gene symbol: Neurog2
Gene name: neurogenin 2
Results
Specimen 1A: embryonic day 12.5; Ascl1tm1Reed/Ascl1+ (more )
Note: Double labeled: red - Neurog2; green - Ascl1 reporter (assay MGI:7855848).
Structure Level Pattern Image Note
TS20: retina Present Regionally restricted 1A Expression domain extended further peripherally than Ascl1.
Specimen 1B: embryonic day 13.5; Ascl1tm1Reed/Ascl1+ (more )
Note: Double labeled: red - Neurog2; green - Ascl1 reporter (assay MGI:7855848).
Structure Level Pattern Image Note
TS21: retina Present Regionally restricted 1B Expression domain extended further peripherally than Ascl1.
Specimen 1G/I/K/L: embryonic day 14.5; Ascl1tm1Reed/Ascl1+ (more )
Note: Triple labeled: red - Neurog2; green - Ascl1 reporter (assay MGI:7855848); white - Olig2 (assay MGI:7855850).
Structure Level Pattern Image Note
TS22: retina Present 1I , 1G , 1K , 1L Cells that expressed Ascl1-GFP only; Olig2 only; Neurog2 only; Ascl1-GFP and Olig2; Ascl1-GFP and Neurog2; Olig2 and Neurog2; and all three transcription factors were observed.
Specimen 1M: embryonic day 14.5; Ascl1tm1Reed/Ascl1tm1Reed (more )
Note: Triple labeled: red - Neurog2; green - Ascl1 reporter (assay MGI:7855848); white - Olig2 (assay MGI:7855850).
Structure Level Pattern Image Note
TS22: retina Present 1M Similar as in the control.
Specimen S1B/D: embryonic day 18.5; Ascl1tm1Reed/Ascl1+ (more )
Note: Triple labeled: red - Neurog2; green - Ascl1 reporter (assay MGI:7855848); white - Olig2 (assay MGI:7855850).
Structure Level Pattern Image Note
TS26: retina Present S1D , S1B Cells that expressed Ascl1-GFP only; Olig2 only; Neurog2 only; Ascl1-GFP and Olig2; Ascl1-GFP and Neurog2; Olig2 and Neurog2; and all three transcription factors were observed.
Specimen S1F/H: embryonic day 18.5; Ascl1tm1Reed/Ascl1tm1Reed (more )
Note: Triple labeled: red - Neurog2; green - Ascl1 reporter (assay MGI:7855848); white - Olig2 (assay MGI:7855850).
Structure Level Pattern Image Note
TS26: retina Present S1F , S1H Mutant animals had each population as in the control, but there were fewer Ascl1-GFP+, Neurog2+ and Olig2+ cells seen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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