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Gene Expression Data
Assay Details
Assay
Reference: J:208419 Basta JM, et al., Sall1 balances self-renewal and differentiation of renal progenitor cells. Development. 2014 Mar;141(5):1047-58
Assay type: RT-PCR
MGI Accession ID: MGI:7855518
Gene symbol: Sema5a
Gene name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Probe: Sema5a-pF, Sema5a-pR qRT
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Rpl19 or Hprt1.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
E11.5 +/- E11.5 TS19: metanephros Present Not Specified; total RNA involves: 129P2/OlaHsd * ICR Sall1tm3Ryn/Sall1+Not Specified
E12.5 +/- E12.5 TS20: metanephros Present Not Specified; total RNA involves: 129P2/OlaHsd * ICR Sall1tm3Ryn/Sall1+Not Specified
E11.5 +/- E11.5 TS19: metanephros Present Not Specified; total RNA involves: 129P2/OlaHsd * ICR Sall1tm3Ryn/Sall1+Not Specified
E12.5 -/- E12.5 TS20: metanephros Present Not Specified; total RNA involves: 129P2/OlaHsd * ICR Sall1tm3Ryn/Sall1tm3RynNot Specified
Notes:
(a) Genes that normally go up in expression in the renal vesicle, including Sema5a, show a greater increase in expression in the mutant kidney compared to the change observed in the control from E11.5 to E12.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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