Spi1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:39856 Scott EW, et al., PU.1 functions in a cell-autonomous manner to control the differentiation of multipotential lymphoid-myeloid progenitors. Immunity. 1997 Apr;6(4):437-47
Assay type:	Western blot
MGI Accession ID:	MGI:7798773
Gene symbol:	Spi1
Gene name:	Spi-1 proto-oncogene

Antibody:

Anti-PU.1

Results

Image: 1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Genetic Background	Mutant Allele(s)	Sex
PU.1	In vitro data: no data stored
PUl1 1-198	In vitro data: no data stored
Thymus +/+	P	TS28: thymus	Absent	involves: 129S/SvEv		Not Specified
Spleen +/+	P	TS28: spleen	Present	involves: 129S/SvEv		Not Specified
Spleen +/-	P	TS28: spleen	Present (b)	involves: 129S/SvEv	Spi1^tm1Sing/Spi1⁺	Not Specified
Fetal Liver +/+	Tissue age not specified: no data stored
Fetal Liver +/-	Tissue age not specified: no data stored
Fetal Liver -/-	Tissue age not specified: no data stored

Notes:
(a) No PU.1 protein product was detectable in the fetal liver of homozygous mutant embryos and reduced levels of full-length PU.1 protein were observed in the fetal liver of heterozygotes.
(b) Heterozygous mice reproducibly showed reduced levels of PU.1 protein in spleen.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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