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Gene Expression Data
Assay Details
Assay
Reference: J:355681 Dias Mirandela M, et al., Two-factor authentication underpins the precision of the piRNA pathway. Nature. 2024 Oct;634(8035):979-985
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7787114
Gene symbol: Piwil4
Gene name: piwi-like RNA-mediated gene silencing 4
Antibody: anti-Miwi2
Detection system: Secondary antibody
Results
Specimen 1a PBS: embryonic day 16.5; Spocd1em2Doca/Spocd1+ (more )
Note: Double labeled: green - Piwil4; red - Spocd1 reporter (assay MGI:7787142).
Structure Level Pattern Image Note
TS24: germ cell of testis Present 1a upper MIWI2 , 1a upper merge A considerable nuclear fraction was observed.
Specimen 4e WT: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present 4e left
Specimen 4e mutant: embryonic day 16.5; Spocd1em3Doca/Spocd1em3Doca (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present 4e right Similar as in the wild-type.
Specimen EDF1a PBS: embryonic day 16.5; Spocd1em2Doca/Spocd1+ (more )
Note: Double labeled: green - Piwil4; red - Spocd1 reporter (assay MGI:7787142).
Structure Level Pattern Image Note
TS24: germ cell of testis Present EDF1a PBS merge , EDF1a PBS MIWI A considerable nuclear fraction was observed.
Specimen EDF6g wild-type: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present EDF6g left
Specimen EDF6g mutant: embryonic day 16.5; Spocd1em3Doca/Spocd1em3Doca (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present EDF6g right Similar as in the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory