Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:77237
Verhage M, et al., Synaptic assembly of the brain in the absence of neurotransmitter secretion. Science. 2000 Feb 4;287(5454):864-9
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7718351
|
|
Gene symbol:
|
Syn1
|
|
Gene name:
|
synapsin I
|
|
Antibody:
|
anti-pan-synapsin (E028)
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Assay notes:
|
Fixation was with 71.4% saturated picric acid, 23.8% formalin, and 4.8% acetic acid.
|
|
|
Results
|
Specimen
3I:
embryonic day 18.0
(more  )
Note: Data from wild-type and heterozygous embryos were pooled as the control group after pilot experiments revealed no differences between these groups.
Specimen
3I:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Data from wild-type and heterozygous embryos were pooled as the control group after pilot experiments revealed no differences between these groups.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Regionally restricted |
3I
|
Expression tranported to synaptic layer.
|
Specimen
3I':
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Stxbp1tm1Sud/Stxbp1tm1Sud
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Analysis Tools
