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Gene Expression Data
Assay Details
Assay
Reference: J:250104 Flodby P, et al., The 78-kD Glucose-Regulated Protein Regulates Endoplasmic Reticulum Homeostasis and Distal Epithelial Cell Survival during Lung Development. Am J Respir Cell Mol Biol. 2016 Jul;55(1):135-49
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7704204
Gene symbol: Hsp90b1
Gene name: heat shock protein 90, beta (Grp94), member 1
Antibody: anti-GRP94 (9G10 SPA-850)
Detection system: Secondary antibody
Results
Specimen 4C f/f: embryonic day 14.0; Hspa5tm1Alee/Hspa5tm1Alee (more )
Note: Double labeled: green - Hsp90b1; red - Nkx2-1 (assay MGI:7704205).
Structure Cell Type Level Pattern Image Note
TS22: lung epithelium epithelial cell of lung Ambiguous 4C f/f Absent or weak expression.
Specimen 4C cKO: embryonic day 14.0; Hspa5tm1Alee/Hspa5tm1Alee, Tg(Sftpc-cre)1Blh/0 (more )
Note: Conditional mutant. Double labeled: green - Hsp90b1; red - Nkx2-1 (assay MGI:7704205).
Structure Cell Type Level Pattern Image Note
TS22: lung epithelium epithelial cell of lung Present 4C cKO Increased expression in Nkx2-1+ epithelial cells.
Specimen 4G f/f: embryonic day 18.0; Hspa5tm1Alee/Hspa5tm1Alee (more )
Structure Cell Type Level Pattern Image Note
TS26: terminal bronchiole epithelium epithelial cell of lung Ambiguous 4 cont_G f/f Absent or weak expression.
Specimen 4G cKO: embryonic day 18.0; Hspa5tm1Alee/Hspa5tm1Alee, Tg(Sftpc-cre)1Blh/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS26: terminal bronchiole epithelium epithelial cell of lung Strong 4 cont_G cKO Increased expression in epithelial cells lining the saccules in distal lung compared with the controls.
Specimen E5A: embryonic day 18.0; Hspa5tm1Alee/Hspa5tm1Alee, Tg(Sftpc-cre)1Blh/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS26: lung epithelium pulmonary alveolar epithelial cell Present Regionally restricted E5A Increased expression in alveolar epithelial cells, but not in bronchiolar epithelial cells in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory