Gene Expression Data
Assay Details
Assay
|
Reference:
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J:306034
Troy TC, et al., Re-assessing K15 as an epidermal stem cell marker. Stem Cell Rev Rep. 2011 Nov;7(4):927-34
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7628029
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Gene symbol:
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Krt15
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Gene name:
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keratin 15
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Antibody:
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anti-K15 (UC55)
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
1a:
postnatal newborn
(more )
Specimen
1a:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal newborn
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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Bouin's Fixative
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: oral epithelium |
Present |
Regionally restricted |
1a
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Expression mostly restricted to undifferentiated basal cells of keratinizing oral epithelium of roof of mouth.
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Specimen
1b:
postnatal newborn
(more )
Specimen
1b:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: oral epithelium |
Present |
Regionally restricted |
1b
|
Expression in many layers of non-keratinizing oral epithelium.
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Specimen
1c:
postnatal newborn
(more )
Specimen
1c:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: tongue keratinized epithelium |
Present |
Regionally restricted |
1c
|
Expression localized to undifferentiated basal cells of keratinized stratified squamous epithelium on dorsal surface of tongue.
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Specimen
1d:
postnatal newborn
(more )
Specimen
1d:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: tongue epithelium |
Present |
Regionally restricted |
1d
|
Expression in continuous non-keratinized epithelium on ventral surface of tongue.
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Specimen
1e:
postnatal newborn
(more )
Specimen
1e:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
1f:
postnatal newborn
(more )
Specimen
1f:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: vibrissa follicle |
Present |
Regionally restricted |
1f
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Expression in several layers of whisker follicle.
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Specimen
1h:
postnatal newborn
(more )
Specimen
1h:
(close )
Genetic Background:
|
CD-1
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Age:
|
postnatal newborn
|
Sex:
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Not Specified
|
Type:
|
section
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Fixation:
|
Bouin's Fixative
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: proximal nail fold |
Present |
Regionally restricted |
1h
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Expression only in basal compartment of proximal nail fold.
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
Bouin's Fixative
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Embedding:
|
Paraffin
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Specimen
2a:
embryonic day 12.5
(more )
Specimen
2a:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: epidermis basal layer |
Present |
|
2a
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Expression in basal cell layer of ectoderm and overlying periderm.
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TS20: periderm |
Present |
|
2a
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Expression in basal cell layer of ectoderm and overlying periderm.
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Specimen
2b:
embryonic day 13.5
(more )
Specimen
2b:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: epidermis basal layer |
Present |
|
2b
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Expression in basal cell layer of ectoderm and overlying periderm.
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TS21: periderm |
Present |
|
2b
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Expression in basal cell layer of ectoderm and overlying periderm.
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TS21: epidermis |
Present |
Regionally restricted |
2b
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No expression in intermediate cells between basal layer and periderm.
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Specimen
2c:
embryonic day 14.5
(more )
Specimen
2c:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
Bouin's Fixative
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: epidermis basal layer |
Present |
Regionally restricted |
2c
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Expression in basal cell layer of ectoderm and overlying periderm.
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TS22: periderm |
Present |
Regionally restricted |
2c
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Expression in basal cell layer of ectoderm and overlying periderm.
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TS22: epidermis |
Present |
Regionally restricted |
2c
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No expression in an intermediate layer of cells between basal layer and periderm.
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TS22: hair epidermal placode |
Absent |
|
2c
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No expression where initial peg formation is evident.
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Specimen
2d:
embryonic day 15.5
(more )
Specimen
2d:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
Bouin's Fixative
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: epidermis basal layer |
Present |
|
2d
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Expression in basal cell layer of ectoderm.
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TS23: periderm |
Very strong |
|
2d
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Very strong expression in overlying periderm.
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TS23: hair germ |
Present |
Regionally restricted |
2d
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Expression in cells emerging into peg of developing hair follicle.
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Specimen
2e:
embryonic day 16.5
(more )
Specimen
2e:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: epidermis basal layer |
Present |
|
2e
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Expression in basal cell layer of ectoderm.
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TS24: periderm |
Very strong |
|
2e
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Very strong expression in overlying periderm.
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TS24: hair peg |
Present |
|
2e
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Expression in cells emerging into peg of developing hair follicle.
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Specimen
2f:
embryonic day 17.5
(more )
Specimen
2f:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: epidermis basal layer |
Present |
|
2f
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Expression in basal cell layer of ectoderm.
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TS25: hair peg |
Present |
Regionally restricted |
2f
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As hair peg elongates, leading edge of expression restricted to outer root sheath.
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Specimen
2g:
embryonic day 18.5
(more )
Specimen
2g:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: epidermis basal layer |
Present |
|
2g
|
Expression in basal cell layer of ectoderm.
|
TS26: hair peg |
Present |
Regionally restricted |
2g
|
As hair peg elongates, leading edge of expression restricted to outer root sheath.
|
Specimen
2h:
postnatal newborn
(more )
Specimen
2h:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: epidermis basal layer |
Present |
|
2h
|
Expression in basal cell layer of ectoderm.
|
TS27: hair outer root sheath |
Present |
Regionally restricted |
2h
|
As hair peg elongates, leading edge of expression restricted to outer root sheath.
|
TS27: hair peg |
Present |
Regionally restricted |
2h
|
As hair peg elongates, leading edge of expression restricted to outer root sheath.
|
Specimen
2i:
postnatal adult
(more )
Specimen
2i:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
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Embedding:
|
Paraffin
|
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