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Gene Expression Data
Assay Details
Assay
Reference: J:346903 Saito S, et al., Functional analysis of a first hindlimb positioning enhancer via Gdf11 expression. Front Cell Dev Biol. 2024;12:1302141
Assay type: RT-PCR
MGI Accession ID: MGI:7627422
Gene symbol: Gdf11
Gene name: growth differentiation factor 11
Probe: mGdf11-F, mGdf11-R qRT
Probe preparation: labelled with other - see notes
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh. RT-qPCR was performed with TB Green.
Results
Image: 2G E9.5 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
+/+ E9.5 TS15: embryo Present Not Specified Not Specified Not Specified Sample was taken from the posterior tissues containing the presomitic mesoderm.
+/delta HCR E9.5 TS15: embryo Present (a) Not Specified involves: C57BL/6J * DBA/2 Rr476em1Tkto/Rr476+Not Specified Sample was taken from the posterior tissues containing the presomitic mesoderm.
delta HCR/delta HCR E9.5 TS15: embryo Weak (b) Not Specified involves: C57BL/6J * DBA/2 Rr476em1Tkto/Rr476em1TktoNot Specified Sample was taken from the posterior tissues containing the presomitic mesoderm.
Notes:
(a) Heterozygous expression level was reduced to ~70% of wild type.
(b) Mutant expression was reduced to approximately 30% that of wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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