Gene Expression Data
Assay Details
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Assay
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Reference:
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J:345410
Wang Y, et al., TMEM216 promotes primary ciliogenesis and Hedgehog signaling through the SUFU-GLI2/GLI3 axis. Sci Signal. 2024 Jan 23;17(820):eabo0465
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7618269
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Gene symbol:
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Tubg1
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Gene name:
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tubulin, gamma 1
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Antibody:
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anti-gamma-tubulin (T3320)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Assay notes:
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The specificity of the antibody used was not detailed; both family members have been annotated.
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Results
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Specimen
2A +/+:
embryonic day 17.5
(more  )
Specimen
2A +/+:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: metanephros |
Present |
Regionally restricted |
2A +/+
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Expression is in centrioles.
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Specimen
2A -/-:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Tmem216em1Cya/Tmem216em1Cya
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: metanephros |
Present |
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2A -/-
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In this mutant the kidney cilia had shorter lengths and abnormal morphology compared to their wild-type littermates.
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Analysis Tools