Gene Expression Data
Assay Details
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Assay
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Reference:
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J:302420
Chleilat E, et al., TGF-beta Signaling Regulates SLC8A3 Expression and Prevents Oxidative Stress in Developing Midbrain Dopaminergic and Dorsal Raphe Serotonergic Neurons. Int J Mol Sci. 2020 Apr 15;21(8):2735
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7616866
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Gene symbol:
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Slc8a3
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Gene name:
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solute carrier family 8 (sodium/calcium exchanger), member 3
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Antibody:
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anti-SLC8A3
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
1A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ
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Mutant Allele(s):
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Tgfbr2tm1.2Hlm/Tgfbr2tm1.2Hlm
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
1a1
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1A
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1a2
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Expressed broadly in the ventral midbrain. Moderate to strong intensity was detected in neurons of the substantia nigra pars compacta and ventral tegmental area.
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Specimen
1B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ
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Mutant Allele(s):
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En1tm2(cre)Wrst/En1+
Tgfbr2tm1.2Hlm/Tgfbr2tm1.2Hlm
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
1b2
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1B
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1b1
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Expressed in the ventral midbrain, with decreased cell number of positive neurons and labeling intensity of the substantia nigra pars compacta and ventral tegmental area compared with the control.
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Specimen
1C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ
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Mutant Allele(s):
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Tgfbr2tm1.2Hlm/Tgfbr2tm1.2Hlm
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: hindbrain |
serotonergic neuron |
Present |
Regionally restricted |
1c2
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1C
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1c1
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Expressed broadly in the ventral hindbrain. Moderate to strong intensity was detected in neurons of all subpopulations of the dorsal raphe.
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Specimen
1D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ
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Mutant Allele(s):
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En1tm2(cre)Wrst/En1+
Tgfbr2tm1.2Hlm/Tgfbr2tm1.2Hlm
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: hindbrain |
serotonergic neuron |
Present |
Regionally restricted |
1d1
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1d2
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1D
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Expressed in the ventral hindbrain, with decreased cell number and labeling intensity of positive serotonergic neurons of the dorsal raphe compared with the control.
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)
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