Lgr6 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:262420 Szenker-Ravi E, et al., RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6. Nature. 2018 May;557(7706):564-569
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7606802
Gene symbol:	Lgr6
Gene name:	leucine-rich repeat-containing G protein-coupled receptor 6

Reporter:	GFP
Antibody:	anti-GFP

Results

Specimen 2i Triple KO (left): embryonic day 14.5; Lgr4^{tm1(cre/ERT2)Cle}/Lgr4^{tm1(cre/ERT2)Cle}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa}, Lgr6^{tm2.1(cre/ERT2)Cle}/Lgr6^{tm2.1(cre/ERT2)Cle} (more

)
Note: Double labeled: green - Lgr6 and Lgr4 and Lgr5 reporter; red - Vim (assay MGI:7596300).

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	2i Lgr4/5/6 -/- Vimentin	Expression in single layer of Vim-expressing cells adjacent to Cdh1-expressing epithelium.

Specimen 2i Triple KO inset (left): embryonic day 14.5; Lgr4^{tm1(cre/ERT2)Cle}/Lgr4^{tm1(cre/ERT2)Cle}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa}, Lgr6^{tm2.1(cre/ERT2)Cle}/Lgr6^{tm2.1(cre/ERT2)Cle} (more

)
Note: Double labeled: green - Lgr6 and Lgr4 and Lgr5 reporter; red - Vim (assay MGI:7596300).

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	2i Lgr4/5/6 -/- Vimentin inset	Expression in single layer of Vim-expressing cells adjacent to Cdh1-expressing epithelium.

Specimen 2i Triple KO (right): embryonic day 14.5; Lgr4^{tm1(cre/ERT2)Cle}/Lgr4^{tm1(cre/ERT2)Cle}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa}, Lgr6^{tm2.1(cre/ERT2)Cle}/Lgr6^{tm2.1(cre/ERT2)Cle} (more

)
Note: Double labeled: green - Lgr6 and Lgr4 and Lgr5 reporter; red - Cdh1 (assay MGI:7596301).

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	2i Lgr4/5/6 -/- E-cadherin	Expression in single layer of Vim-expressing cells adjacent to Cdh1-expressing epithelium.

Specimen 2i Triple KO inset (right): embryonic day 14.5; Lgr4^{tm1(cre/ERT2)Cle}/Lgr4^{tm1(cre/ERT2)Cle}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa}, Lgr6^{tm2.1(cre/ERT2)Cle}/Lgr6^{tm2.1(cre/ERT2)Cle} (more

)
Note: Double labeled: green - Lgr6 and Lgr4 and Lgr5 reporter; red - Cdh1 (assay MGI:7596301).

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	2i Lgr4/5/6 -/- E-cadherin inset	Expression in single layer of Vim-expressing cells adjacent to Cdh1-expressing epithelium.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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