Probe:	Grb10 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	2G	Expressed in a small line of cells partially overlapped with the dorsal and rostral mesodiencephalic dopamine neuronal field. Slightly higher transcript levels were observed in the ventricular and intermediate zones.
TS22: midbrain ventricular layer	Present	Regionally restricted	2G	Expression intermingled with the most dorsal TH expression.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	2L	Expressed in a small line of cells partially overlapped with the dorsal and rostral mesodiencephalic dopamine neuronal field. Slightly higher transcript levels were observed in the ventricular and intermediate zones.
TS22: midbrain ventricular layer	Present	Regionally restricted	2L	Expression intermingled with the most dorsal TH expression.

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Present		2P

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Present	Regionally restricted	3F	Clearly co-localized with TH in the medial region.

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Present	Regionally restricted	3O	Clearly co-localized with TH in the medial region.
TS22: midbrain	Present	Regionally restricted	3O	Expressed in two small positive lines directly ventral to the ventricular zone and lateral to the dorsal-medial mesodiencephalic dopamine neuronal area.

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Present	Regionally restricted	3X	Clearly co-localized with TH in the medial region.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5EE

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5FF , 5FF' , 5FF"

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5GG , 5GG'

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5HH

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5II

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5JJ	Slightly lower expression in the dorsal TH domain and in the ventricular zone. The line of cells highly expressing Grb10 dorsally along the entire medial mesodiencephalic dopamine neuronal area was clearly affected.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5KK" , 5KK' , 5KK	Slightly lower expression in the dorsal TH domain and in the ventricular zone. The line of cells highly expressing Grb10 dorsally along the entire medial mesodiencephalic dopamine neuronal area was clearly affected.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5LL' , 5LL	Slightly lower expression in the dorsal TH domain and in the ventricular zone. The line of cells highly expressing Grb10 dorsally along the entire medial mesodiencephalic dopamine neuronal area was clearly affected.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5MM	Slightly lower expression in the dorsal TH domain and in the ventricular zone. The line of cells highly expressing Grb10 dorsally along the entire medial mesodiencephalic dopamine neuronal area was clearly affected.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5NN	Slightly lower expression in the dorsal TH domain and in the ventricular zone. The line of cells highly expressing Grb10 dorsally along the entire medial mesodiencephalic dopamine neuronal area was clearly affected.