Antibody:	anti-GRP78
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: gut endoderm	Strong		2A
TS15: heart	Strong		2A
TS15: somite	Strong		2A
TS15: surface ectoderm	Strong		2A
TS15: future brain	Strong	Regionally restricted	2A	Expression is in germinal cells lining the neural tube.
TS15: neural tube	Strong	Regionally restricted	2A	Expression is in germinal cells lining the neural tube.

Structure	Level	Pattern	Image	Note
TS15: atrium cardiac muscle	Present		2B
TS15: primitive ventricle cardiac muscle	Present		2B
TS15: cardiac jelly	Absent		2B
TS15: endocardial cushion	Absent		2B
TS15: endocardial lining	Absent		2B
TS15: pericardium	Absent		2B
TS15: visceral pericardium	Absent		2B

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Present		2C
TS15: neural tube	Present	Regionally restricted	2C	Expression is in germinal cells lining the neural tube.

Structure	Level	Pattern	Image	Note
TS17: atrium cardiac muscle	Present		2G
TS17: primitive ventricle cardiac muscle	Present		2G
TS17: cardiac jelly	Absent		2G
TS17: endocardial cushion	Absent		2G
TS17: endocardial lining	Absent		2G
TS17: pericardium	Absent		2G
TS17: visceral pericardium	Absent		2G

Structure	Level	Pattern	Image	Note
TS19: atrium cardiac muscle	Present		2H
TS19: ventricle cardiac muscle	Present		2H

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Present		2I
TS20: ventricle cardiac muscle	Present		2I
TS20: endocardial cushion	Absent		2I
TS20: endocardial lining	Absent		2I
TS20: pericardium	Absent		2I
TS20: visceral pericardium	Absent		2I

Structure	Level	Pattern	Image	Note
TS21: atrium cardiac muscle	Present		2J
TS21: ventricle cardiac muscle	Present		2J
TS21: atrioventricular valve	Absent		2J
TS21: endocardial cushion	Absent		2J
TS21: endocardial lining	Absent		2J
TS21: pericardium	Absent		2J
TS21: visceral pericardium	Absent		2J

Structure	Level	Pattern	Image	Note
TS21: atrium cardiac muscle	Present		2K
TS21: ventricle cardiac muscle	Present	Non-Uniform	2K	Expression is stronger in the inner spongy layer compared to the outer compact layer.
TS21: atrioventricular valve	Absent		2K
TS21: endocardial cushion	Absent		2K
TS21: endocardial lining	Absent		2K
TS21: pericardium	Absent		2K
TS21: visceral pericardium	Absent		2K