Probe:	Lmo4 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Weak		1A
TS20: corpus striatum	Present		1A	Expressed in the striatum in the ventral cortical region.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Present		1B
TS23: hippocampus	Present		1B

Structure	Level	Pattern	Image	Note
TS27: cortical plate	Strong	Regionally restricted	1C	Strongly expressed in anterior and posterior regions with clear boundaries but not the medial area in between. Mostly expressed in postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	1D	While the gap between anterior and posterior expression was still detectable, expression emerged in layer 3/4 in the medial cortical region.
TS28: cortical layer III	Present	Widespread	1D
TS28: cortical layer IV	Present	Widespread	1D

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	1E	Expressed in the deeper and upper layers across the entire cortex.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Strong	Regionally restricted	S1A	Strongly expressed in anterior (presumptive motor cortex) and posterior (presumptive visual cortex) regions with clear boundaries, but no or very low expression in the medial area (presumptive somatosensory cortex) in between.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	S5A	Strongly expressed in the most anterior cortex. Weak expression was also detected in the most posterior region. Expressed in the striatum.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted	S5B	Expressed in the anterior and posterior regions.
TS23: cerebellum	Present		S5B
TS23: corpus striatum	Present		S5B
TS23: hindbrain	Present		S5B
TS23: hippocampus	Present		S5B

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebellum Purkinje cell cluster	Purkinje cell	Present		S5C
TS23: cerebellum		Present		S5C	Highest expression in Purkinje cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		S5D
TS28: cerebellum		Present		S5D	Highest expression in Purkinje cells.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Regionally restricted	S5E	Strong expression in a subset of motor neurons.