Gene Expression Data
Assay Details
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Assay
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Reference:
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J:316482
Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7576031
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Gene symbol:
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Slc6a3
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Gene name:
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solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
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Probe:
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Dat cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5B +/+ lateral to medial 1:
embryonic day 14.5
(more  )
Specimen
5B +/+ lateral to medial 1:
(close  )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5B +/+ 1
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Expressed in a rostral subset of the mesodiencephalic dopaminergic neurons.
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Specimen
5B +/+ lateral to medial 2:
embryonic day 14.5
(more )
Specimen
5B +/+ lateral to medial 2:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5B +/+ 2
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Expressed in a rostral subset of the mesodiencephalic dopaminergic neurons.
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Specimen
5B +/+ lateral to medial 3:
embryonic day 14.5
(more )
Specimen
5B +/+ lateral to medial 3:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5B +/+ 3
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Expressed in a rostral subset of the mesodiencephalic dopaminergic neurons.
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Specimen
5B +/+ lateral to medial 4:
embryonic day 14.5
(more )
Specimen
5B +/+ lateral to medial 4:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5B +/+ 4
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Expressed in a rostral subset of the mesodiencephalic dopaminergic neurons.
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Specimen
5B +/+ lateral to medial 5:
embryonic day 14.5
(more )
Specimen
5B +/+ lateral to medial 5:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5B +/+ 5
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Expressed in a rostral subset of the mesodiencephalic dopaminergic neurons.
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Specimen
5B LacZ/LacZ lateral to medial 1:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5B LacZ/LacZ 1
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Decreased expression in mesodiencephalic dopaminergic neuronal region, compared with the wild-type. Loss of expression was highly specific to the most caudomedial region.
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Specimen
5B LacZ/LacZ lateral to medial 2:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5B LacZ/LacZ 2
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Decreased expression in mesodiencephalic dopaminergic neuronal region, compared with the wild-type. Loss of expression was highly specific to the most caudomedial region.
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Specimen
5B LacZ/LacZ lateral to medial 3:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5B LacZ/LacZ 3
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Decreased expression in mesodiencephalic dopaminergic neuronal region, compared with the wild-type. Loss of expression was highly specific to the most caudomedial region.
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Specimen
5B LacZ/LacZ lateral to medial 4:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5B LacZ/LacZ 4
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Decreased expression in mesodiencephalic dopaminergic neuronal region, compared with the wild-type. Loss of expression was highly specific to the most caudomedial region.
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Specimen
5B LacZ/LacZ lateral to medial 5:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5B LacZ/LacZ 5
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Decreased expression in mesodiencephalic dopaminergic neuronal region, compared with the wild-type. Loss of expression was highly specific to the most caudomedial region.
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Specimen
S1A WT rostral to caudal 1:
postnatal adult
(more )
Specimen
S1A WT rostral to caudal 1:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1A WT rostral to caudal 2:
postnatal adult
(more )
Specimen
S1A WT rostral to caudal 2:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1A WT rostral to caudal 3:
postnatal adult
(more )
Specimen
S1A WT rostral to caudal 3:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1A WT rostral to caudal 4:
postnatal adult
(more )
Specimen
S1A WT rostral to caudal 4:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1A KO rostral to caudal 1:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
midbrain dopaminergic neuron |
Present |
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S1A KO 1
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Similar as in the wild-type.
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Specimen
S1A KO rostral to caudal 2:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
midbrain dopaminergic neuron |
Present |
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S1A KO 2
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Similar as in the wild-type.
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Specimen
S1A KO rostral to caudal 3:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
midbrain dopaminergic neuron |
Present |
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S1A KO 3
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Similar as in the wild-type.
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Specimen
S1A KO rostral to caudal 4:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
midbrain dopaminergic neuron |
Present |
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S1A KO 4
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Similar as in the wild-type.
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