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Gene Expression Data
Assay Details
Assay
Reference: J:143529 Ichise H, et al., Phospholipase C{gamma}2 is necessary for separation of blood and lymphatic vasculature in mice. Development. 2009 Jan;136(2):191-5
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7574218
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-CD31
Detection system: Biotinylated secondary antibody/[Strept]avidin
Results
Specimen 2A/D: embryonic day 13.5; Plcg2tm1Ichi/Plcg2+ (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:7574217).
Structure Cell Type Level Pattern Image Note
TS21: blood vessel endothelium vein endothelial cell Present 2A , 2D
TS21: artery endothelium endothelial cell of artery Present 2A
Specimen 2B/E: embryonic day 13.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:7574217).
Structure Cell Type Level Pattern Image Note
TS21: artery endothelium endothelial cell of artery Present 2B
TS21: blood vessel endothelium vein endothelial cell Present 2B , 2E
TS21: lymph sac blood vessel endothelial cell Present 2E , 2B Blood-filled lymph sacs consisted of both Lyve1+ lymphatic and CD31+ blood endothelial cells. The endothelial cells remained close to those of the cardinal veins.
Specimen 2C/F: embryonic day 13.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:7574217).
Structure Cell Type Level Pattern Image Note
TS21: artery endothelium endothelial cell of artery Present 2C
TS21: blood vessel endothelium vein endothelial cell Present Regionally restricted 2F , 2C Fusion between lymph sac and blood vessel was seen.
Specimen 3B: embryonic day 13.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: red - Pecam1; green - Plcg2 reporter (assay MGI:7574221).
Structure Cell Type Level Pattern Image Note
TS21: blood vessel endothelium vein endothelial cell Present 3B right , 3B middle
Specimen 3C: embryonic day 14.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: red - Pecam1; green - Plcg2 reporter (assay MGI:7574221).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelium blood vessel endothelial cell Present 3C right , 3C middle
Specimen 3Ea/c: embryonic day 13.5; Plcg2tm1Ichi/Plcg2+ (more )
Note: Double labeled: red - Pecam1; green - Plcg2 reporter (assay MGI:7574221).
Structure Cell Type Level Pattern Image Note
TS21: blood vessel endothelium vein endothelial cell Present 3Ec , 3Ea
Specimen 3Eb/d: embryonic day 13.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: red - Pecam1; green - Plcg2 reporter (assay MGI:7574221).
Structure Cell Type Level Pattern Image Note
TS21: blood vessel endothelium vein endothelial cell Present 3Ed , 3Eb
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory