About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:143529 Ichise H, et al., Phospholipase C{gamma}2 is necessary for separation of blood and lymphatic vasculature in mice. Development. 2009 Jan;136(2):191-5
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7574218
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-CD31
Detection system: Biotinylated secondary antibody/[Strept]avidin
Results
Specimen 2A/D: embryonic day 13.5; Plcg2tm1Ichi/Plcg2+ (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:7574217).
Structure Cell Type Level Pattern Image Note
TS21: blood vessel endothelium vein endothelial cell Present 2A , 2D
TS21: artery endothelium endothelial cell of artery Present 2A
Specimen 2B/E: embryonic day 13.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:7574217).
Structure Cell Type Level Pattern Image Note
TS21: artery endothelium endothelial cell of artery Present 2B
TS21: blood vessel endothelium vein endothelial cell Present 2B , 2E
TS21: lymph sac blood vessel endothelial cell Present 2E , 2B Blood-filled lymph sacs consisted of both Lyve1+ lymphatic and CD31+ blood endothelial cells. The endothelial cells remained close to those of the cardinal veins.
Specimen 2C/F: embryonic day 13.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: red - Pecam1; green - Lyve1 (assay MGI:7574217).
Structure Cell Type Level Pattern Image Note
TS21: artery endothelium endothelial cell of artery Present 2C
TS21: blood vessel endothelium vein endothelial cell Present Regionally restricted 2F , 2C Fusion between lymph sac and blood vessel was seen.
Specimen 3B: embryonic day 13.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: red - Pecam1; green - Plcg2 reporter (assay MGI:7574221).
Structure Cell Type Level Pattern Image Note
TS21: blood vessel endothelium vein endothelial cell Present 3B right , 3B middle
Specimen 3C: embryonic day 14.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: red - Pecam1; green - Plcg2 reporter (assay MGI:7574221).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelium blood vessel endothelial cell Present 3C right , 3C middle
Specimen 3Ea/c: embryonic day 13.5; Plcg2tm1Ichi/Plcg2+ (more )
Note: Double labeled: red - Pecam1; green - Plcg2 reporter (assay MGI:7574221).
Structure Cell Type Level Pattern Image Note
TS21: blood vessel endothelium vein endothelial cell Present 3Ec , 3Ea
Specimen 3Eb/d: embryonic day 13.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: red - Pecam1; green - Plcg2 reporter (assay MGI:7574221).
Structure Cell Type Level Pattern Image Note
TS21: blood vessel endothelium vein endothelial cell Present 3Ed , 3Eb
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory