Scg5 RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:123068 Pavel E, et al., Analysis of a new allele of limb deformity (ld) reveals tissue- and age-specific transcriptional effects of the Ld Global Control Region. Int J Dev Biol. 2007;51(4):273-81
Assay type:	RT-PCR
MGI Accession ID:	MGI:7565451
Gene symbol:	Scg5
Gene name:	secretogranin V

Probe:	Sgne1-pF, Sgne1-pR
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Gapdh.

Results

Image: 5_Sgne1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
Het Male	P adult	TS28: metanephros	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1⁺	Male
Mut Male	P adult	TS28: metanephros	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1^ld-Lsk	Male
Het Female	P adult	TS28: metanephros	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1⁺	Female
Mut Female	P adult	TS28: metanephros	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1^ld-Lsk	Female
Het Male	P adult	TS28: lung	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1⁺	Male
Mut Male	P adult	TS28: lung	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1^ld-Lsk	Male
Het Female	P adult	TS28: lung	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1⁺	Female
Mut Female	P adult	TS28: lung	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1^ld-Lsk	Female
Het Male	P adult	TS28: brain	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1⁺	Male
Mut Male	P adult	TS28: brain	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1^ld-Lsk	Male
Het Female	P adult	TS28: brain	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1⁺	Female
Mut Female	P adult	TS28: brain	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1^ld-Lsk	Female
Het Male	P adult	TS28: pituitary gland	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1⁺	Male
Mut Male	P adult	TS28: pituitary gland	Present (a)	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1^ld-Lsk	Male
Het Female	P adult	TS28: pituitary gland	Present	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1⁺	Female
Mut Female	P adult	TS28: pituitary gland	Present (b)	Not Specified; poly-A+ RNA	C.Cg-Fmn1^ld-Lsk	Fmn1^ld-Lsk/Fmn1^ld-Lsk	Female

Notes:
(a) Expression was increased in this mutant compared to male heterozygotes.
(b) Expression was significantly increased in this mutant compared to female heterozygotes.

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