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Gene Expression Data
Assay Details
Assay
Reference: J:233705 Zhao H, et al., Deletions of Retinoblastoma 1 (Rb1) and Its Repressing Target S Phase Kinase-associated protein 2 (Skp2) Are Synthetic Lethal in Mouse Embryogenesis. J Biol Chem. 2016 May 6;291(19):10201-9
Assay type: RT-PCR
MGI Accession ID: MGI:7562462
Gene symbol: Bbc3
Gene name: BCL2 binding component 3
Probe: PUMA pF, PUMA pR
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh.
Results
Image: 5B PUMA ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
Rb1lox/lox E13.5 TS21: brain Present 2.5 µg; total RNA involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3TyjNot Specified
Nes-Cre;Rb1lox/lox E13.5 TS21: brain Present (a) 2.5 µg; total RNA involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Rb1lox/lox;Skp2-/- E13.5 TS21: brain Present (a) 2.5 µg; total RNA involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Skp2tm1Kin/Skp2tm1KinNot Specified
Nes-Cre;Rb1lox/lox;Skp2-/- E13.5 TS21: brain Present (b) 2.5 µg; total RNA involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Skp2tm1Kin/Skp2tm1Kin, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Notes:
(a) Similar as in the Rb1<lox/lox>.
(b) Increased expression compared with the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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