Pax6 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:342123 Sterling NA, et al., P53 independent pathogenic mechanisms contribute to BubR1 microcephaly. Front Cell Dev Biol. 2023;11:1282182
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7548373
Gene symbol:	Pax6
Gene name:	paired box 6

Antibody:	anti-Pax6 (#PRB-278P; #30130)
Detection system:	Secondary antibody

Results

4D WT ©

Specimen 4D PAX6 WT: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		4D WT	Expression in apical radial glia progenitors.

4D Trp53 cKO ©

Specimen 4D PAX6 Trp53 cKO: embryonic day 14.5; Emx1^tm1(cre)Krj/Emx1⁺, Trp53^tm1Brn/Trp53^tm1Brn (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		4D Trp53 cKO

4D BubR1 cKO ©

Specimen 4D PAX6 BubR1 cKO: embryonic day 14.5; Bub1b^tm1Jvd/Bub1b^tm1Jvd, Emx1^tm1(cre)Krj/Emx1⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Weak		4D BubR1 cKO	Expression significantly reduced compared to wild type.

4D dcKO ©

Specimen 4D PAX6 dcKO: embryonic day 14.5; Bub1b^tm1Jvd/Bub1b^tm1Jvd, Emx1^tm1(cre)Krj/Emx1⁺, Trp53^tm1Brn/Trp53^tm1Brn (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		4D dcKO	Expression partially rescued but not to wild type levels.

4H WT ©

Specimen 4H PAX6 WT: embryonic day 14.5 (more

)
Note: Double labeled: red - Pax6; green - BrdU.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		4H WT

4H Trp53 cKO ©

Specimen 4H PAX6 Trp53 cKO: embryonic day 14.5; Emx1^tm1(cre)Krj/Emx1⁺, Trp53^tm1Brn/Trp53^tm1Brn (more

)
Note: Conditional mutant. Double labeled: red - Pax6; green - BrdU.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		4H Trp53 cKO

4H BubR1 cKO ©

Specimen 4H PAX6 BubR1 cKO: embryonic day 14.5; Bub1b^tm1Jvd/Bub1b^tm1Jvd, Emx1^tm1(cre)Krj/Emx1⁺ (more

)
Note: Conditional mutant. Double labeled: red - Pax6; green - BrdU.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Weak		4H BubR1 cKO	Expression reduced compared to wild type.

4H dcKO ©

Specimen 4H PAX6 dcKO: embryonic day 14.5; Bub1b^tm1Jvd/Bub1b^tm1Jvd, Emx1^tm1(cre)Krj/Emx1⁺, Trp53^tm1Brn/Trp53^tm1Brn (more

)
Note: Conditional mutant. Double labeled: red - Pax6; green - BrdU.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		4H dcKO

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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