Gene Expression Data
Assay Details
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Assay
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Reference:
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J:288669
Wedel M, et al., Transcription factor Tcf4 is the preferred heterodimerization partner for Olig2 in oligodendrocytes and required for differentiation. Nucleic Acids Res. 2020 May 21;48(9):4839-4857
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7543406
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Gene symbol:
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Mbp
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Gene name:
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myelin basic protein
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Probe:
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Mbp probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3F:
embryonic day 16.5
(more  )
Specimen
3F:
(close  )
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Genetic Background:
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involves: C3H * C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3G:
embryonic day 18.5
(more )
Specimen
3G:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3H:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Tcf4tm1a(EUCOMM)Wtsi/Tcf4tm1a(EUCOMM)Wtsi
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3I:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Tcf4tm1a(EUCOMM)Wtsi/Tcf4tm1a(EUCOMM)Wtsi
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Trace |
Single cells |
3I
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Expression in few cells, but numbers dramatically reduced compared to control.
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Specimen
9B:
embryonic day 18.5
(more )
Specimen
9B:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
9E:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Tcf4tm1a(EUCOMM)Wtsi/Tcf4+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
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9E
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No difference in number of expressing cells compared to controls.
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Specimen
9H:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J
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Mutant Allele(s):
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Olig2tm1(cre)Tmj/Olig2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
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9H
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No difference in number of expressing cells compared to controls.
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Specimen
9K:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Olig2tm1(cre)Tmj/Olig2+
Tcf4tm1a(EUCOMM)Wtsi/Tcf4+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Weak |
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9K
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Significant reduction in number of expressing cells commpared to controls.
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Specimen
Sox10 het not shown:
embryonic day 18.5; Sox10tm1Weg/Sox10 +
(more )
Note: Data presented in Fig. 10I.
Specimen
Sox10 het not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6J
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Mutant Allele(s):
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Sox10tm1Weg/Sox10+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Data presented in Fig. 10I.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Weak |
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Decreased number of expressing cells compared to controls.
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Specimen
ST double het not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Sox10tm1Weg/Sox10+
Tcf4tm1a(EUCOMM)Wtsi/Tcf4+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Data presented in Fig. 10I.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Weak |
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Even greater decrease in number of expressing cells compared to control.
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Analysis Tools