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Gene Expression Data
Assay Details
Assay
Reference: J:249803 Guerrero-Flores G, et al., Functional determination of the differentiation potential of ventral mesencephalic neural precursor cells during dopaminergic neurogenesis. Dev Biol. 2017 Sep 1;429(1):56-70
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7538897
Gene symbol: Neurog2
Gene name: neurogenin 2
Antibody: Anti-Ngn2
Results
Specimen E11.5: embryonic day 11.5 (more )
Note: Data not shown. Cell counts were graphed in Figure 1A.
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted Expression was detected in neuronal progenitors.

Specimen E12.0: embryonic day 12.0 (more )
Note: Data not shown. Cell counts were graphed in Figure 1A.
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted Expression was detected in neuronal progenitors.

Specimen E12.5: embryonic day 12.5 (more )
Note: Data not shown. Cell counts were graphed in Figure 1A.
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted Expression remained constant at least up to E12.5 in neuronal progenitors.

Specimen 1D left: embryonic day 11.5 (more )
Note: Double labeled: red - Neurog2; green - BrdU.
Structure Level Pattern Image Note
TS20: brain subventricular zone Present 1D BrdU/Neurog2 Two hours after BrdU injection, 5-8% of total BrdU+ cells were also Neurog2+.
TS20: brain ventricular layer Absent 1D BrdU/Neurog2

Specimen 1D right: embryonic day 11.5 (more )
Note: Double labeled: red - Neurog2; green - pHH3.
Structure Level Pattern Image Note
TS20: brain subventricular zone Present 1D PHH3/Neurog2
TS20: brain ventricular layer Absent 1D PHH3/Neurog2

Specimen S1B: embryonic day 12.5 (more )
Note: BrdU at E10.5. Triple labeled: red - Neurog2; purple - Th (assay MGI:7538888); green - BrdU.
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted S1B Merge bottom , S1B Neurog2 Neurog2 co-localized with some BrdU+ cells with weak and strong signal.

Specimen S3A right: embryonic day 11.5 (more )
Note: Double labeled: red - Neurog2; green - Th (assay MGI:7538888).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted S3A right Expression was detected in neuronal progenitors.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory