Gene Expression Data
Assay Details
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Assay
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Reference:
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J:266925
Kim ST, et al., The N-recognin UBR4 of the N-end rule pathway is targeted to and required for the biogenesis of the early endosome. J Cell Sci. 2018 Sep 10;131(17):jcs217646
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7536908
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Gene symbol:
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Eea1
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Gene name:
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early endosome antigen 1
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Results
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Specimen
1C EEA1:
embryonic day 9.5
(more  )
Note: Double labeled: green - Eea1; red - Ubr4 (assay MGI:7536907).
Specimen
1C EEA1:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Eea1; red - Ubr4 (assay MGI:7536907).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: yolk sac endoderm |
Present |
Punctate |
1C (merged)
,
1C EEA1
,
1C (merged) Enlarged
,
1C EEA1 Enlarged
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Punctate expression in early endosomes, more prominent in endoderm than in mesoderm.
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| TS15: yolk sac mesenchyme |
Weak |
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1C EEA1
,
1C (merged)
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1C EEA1 Enlarged
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1C (merged) Enlarged
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Specimen
1D +/+:
embryonic day 9.5
(more )
Specimen
1D +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1D UBR4-/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ubr4tm1.1Ytkw/Ubr4tm1.1Ytkw
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: yolk sac |
Ambiguous |
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1D UBR4-/-
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Authors report expression significantly reduced.
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Specimen
1E Neural tube +/+:
embryonic day 9.5
(more )
Specimen
1E Neural tube +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Punctate |
1E Neural tube +/+ EEA1
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1E Neural tube +/+ Merged
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Specimen
1E Neural tube UBR4-/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ubr4tm1.1Ytkw/Ubr4tm1.1Ytkw
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Ambiguous |
Regionally restricted |
1E Neural tube UBR4-/- EEA1
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1E Neural tube UBR4-/- Merged
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Authors report expression markedly dowregulated and disorganized.
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Specimen
1E Paraxial mesoderm +/+:
embryonic day 9.5
(more )
Specimen
1E Paraxial mesoderm +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: paraxial mesenchyme |
Present |
Punctate |
1E Paraxial mesoderm +/+ EEA1
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1E Paraxial mesoderm +/+ Merged
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Specimen
1E Paraxial mesoderm UBR4-/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ubr4tm1.1Ytkw/Ubr4tm1.1Ytkw
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: paraxial mesenchyme |
Ambiguous |
Regionally restricted |
1E Paraxial mesoderm UBR4-/- Merged
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1E Paraxial mesoderm UBR4-/- EEA1
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Authors report expression markedly dowregulated and disorganized.
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Analysis Tools