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Gene Expression Data
Assay Details
Assay
Reference: J:336094 Zheng H, et al., CHIP inhibits odontoblast differentiation through promoting DLX3 polyubiquitylation and degradation. Development. 2023 May 15;150(10):dev200848
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7532783
Gene symbol: Stub1
Gene name: STIP1 homology and U-Box containing protein 1
Antibody: Anti-Stub1 (ab134064) [EPR4447]
Detection system: Secondary antibody
Assay notes: An in situ proximity ligation assay (PLA) using Stub1 (CHIP) and DLX3 antibodies.
Results
Specimen 5C left: postnatal day 1 (more )
Note: Red fluorescent signals represent the presence of protein interaction.
Structure Cell Type Level Pattern Image Note
TS27: molar mesenchyme mesenchymal cell Present Regionally restricted 5C left Proximity ligation assay indicated that Stub1 interacted with Dlx3 in undifferentiated dental mesenchymal cells.
Specimen 5C right: postnatal day 1 (more )
Note: Red fluorescent signals represent the presence of protein interaction.
Structure Cell Type Level Pattern Image Note
TS27: molar preodontoblast Present 5C right Proximity ligation assay indicated that Stub1 interacted with Dlx3 in preodontoblasts of P1 molars.
Specimen 7Fa: postnatal day 14 (more )
Note: Red fluorescent signals represent the presence of protein interaction.
Structure Cell Type Level Pattern Image Note
TS28: incisor mesenchyme mesenchymal cell Present Regionally restricted 7Fa Proximity ligation assay indicated that Stub1 interacted with Dlx3 in undifferentiated dental mesenchymal cells.
Specimen 7Fb: postnatal day 14 (more )
Note: Red fluorescent signals represent the presence of protein interaction.
Structure Cell Type Level Pattern Image Note
TS28: incisor odontoblast Weak Regionally restricted 7Fb Proximity ligation assay indicated that Stub1-Dlx3 complexes were decreased in secretory odontoblasts.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory