Gene Expression Data
Assay Details
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Assay
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Reference:
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J:339296
Fries M, et al., Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projections. Cell Rep. 2023 Aug 16;42(8):112985
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7525630
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Gene symbol:
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Atoh7
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Gene name:
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atonal bHLH transcription factor 7
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Results
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Specimen
1F E14.5 ATOH7:
embryonic day 14.5
(more  )
Note: Double labeled: magenta - Atoh7; green - Pou3f1 (assay MGI:7525611).
Specimen
1F E14.5 ATOH7:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Atoh7; green - Pou3f1 (assay MGI:7525611).
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Specimen
1F E17.5 ATOH7:
embryonic day 17.5
(more )
Note: Double labeled: magenta - Atoh7; green - Pou3f1 (assay MGI:7525611).
Specimen
1F E17.5 ATOH7:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Atoh7; green - Pou3f1 (assay MGI:7525611).
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Specimen
1F P0 ATOH7:
postnatal day 0
(more )
Note: Double labeled: magenta - Atoh7; green - Pou3f1 (assay MGI:7525611).
Specimen
1F P0 ATOH7:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Atoh7; green - Pou3f1 (assay MGI:7525611).
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Specimen
S5B Pou3f1+/-:
embryonic day 14.5; Pou3f1tm1Mejr/Pou3f1 +
(more )
Specimen
S5B Pou3f1+/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pou3f1tm1Mejr/Pou3f1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S5B Pou3f1-/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pou3f1tm1Mejr/Pou3f1tm1Mejr
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: retina |
retinal ganglion cell |
Present |
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S5B Pou3f1-/- ATOH7
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No difference in number of retinal ganglion cells compared to heterozygous embryos at E14.5.
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Specimen
3A Pou3f1+/- ATOH7:
embryonic day 17.5; Pou3f1tm1Mejr/Pou3f1 +
(more )
Specimen
3A Pou3f1+/- ATOH7:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pou3f1tm1Mejr/Pou3f1+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3A Pou3f1-/- ATOH7:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pou3f1tm1Mejr/Pou3f1tm1Mejr
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: retina |
retinal ganglion cell |
Present |
Regionally restricted |
3A Pou3f1-/- ATOH7
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Increase in propottion of expressing cells in retinal ganglion cell layer in central retina.
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Analysis Tools