Ret In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:339398 Vincent E, et al., Ret deficiency decreases neural crest progenitor proliferation and restricts fate potential during enteric nervous system development. Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2211986120
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7523998
Gene symbol:	Ret
Gene name:	ret proto-oncogene

Reporter:	CFP
Probe:	CFP probe (529381)
Probe preparation:	labelled with beta-galactosidase
Visualized with:	Fluorescence

Results

2E E12.5 CFP/+ ©

Specimen 2E E12.5 CFP/+: embryonic day 12.5; Ret^tm2.1Heno/Ret⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS20: stomach	migratory enteric neural crest cell	Present	Regionally restricted	2E E12.5 CFP/+	Expressing cells have formed a dense network throughout the stomach and all but end of hindgut by E12.5.

2E E12.5 CFP/CFP ©

Specimen 2E E12.5 CFP/CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret^tm2.1Heno (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS20: stomach	migratory enteric neural crest cell	Present	Regionally restricted	2E E12.5 CFP/CFP	Enteric neural crest-derived cells have not left the extrinsically innervating nerve fibers of stomach.

2E E14.5 CFP/+ (left) ©

Specimen 2E E14.5 CFP/+ (left): embryonic day 14.5; Ret^tm2.1Heno/Ret⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: stomach	migratory enteric neural crest cell	Present		2E E14.5 CFP/+ (left)

2E E14.5 CFP/CFP (left) ©

Specimen 2E E14.5 CFP/CFP (left): embryonic day 14.5; Ret^tm2.1Heno/Ret^tm2.1Heno (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: stomach	migratory enteric neural crest cell	Present	Regionally restricted	2E E14.5 CFP/CFP (left)	Enteric neural crest-derived cells have not left the extrinsically innervating nerve fibers of stomach.

2E E14.5 CFP/+ (right) ©

Specimen 2E E14.5 CFP/+ (right): embryonic day 14.5; Ret^tm2.1Heno/Ret⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: hindgut	migratory enteric neural crest cell	Present		2E E14.5 CFP/+ (right)

2E E14.5 CFP/CFP (right) ©

Specimen 2E E14.5 CFP/CFP (right): embryonic day 14.5; Ret^tm2.1Heno/Ret^tm2.1Heno (more

)

Structure	Level	Pattern	Image	Note
TS22: hindgut	Ambiguous	Regionally restricted	2E E14.5 CFP/CFP (right)	Enteric neural crest-derived cells have not left the extrinsically innervating nerve fibers of stomach.

3E CFP/+ (merged) ©

3E CFP/+ CFP ©

Specimen 3E CFP/+ CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret⁺ (more

)
Note: Double labeled: green - Ret reporter; magenta - Cartpt (assay MGI:7524011).

Structure	Level	Pattern	Image	Note
TS20: stomach	Present		3E CFP/+ (merged) , 3E CFP/+ CFP

3E CFP/CFP (merged) ©

3E CFP/CFP CFP ©

Specimen 3E CFP/CFP CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret^tm2.1Heno (more

)
Note: Double labeled: green - Ret reporter; magenta - Cartpt (assay MGI:7524011).

Structure	Level	Pattern	Image	Note
TS20: stomach	Present		3E CFP/CFP CFP , 3E CFP/CFP (merged)

3F CFP/+ (merged) ©

3F CFP/+ CFP ©

Specimen 3F CFP/+ CFP: embryonic day 14.5; Ret^tm2.1Heno/Ret⁺ (more

)
Note: Double labeled: green - Ret reporter; magenta - Cartpt (assay MGI:7524011).

Structure	Level	Pattern	Image	Note
TS22: hindgut	Present		3F CFP/+ (merged) , 3F CFP/+ CFP

3F CFP/CFP (merged) ©

3F CFP/CFP CFP ©

Specimen 3F CFP/CFP CFP: embryonic day 14.5; Ret^tm2.1Heno/Ret^tm2.1Heno (more

)
Note: Double labeled: green - Ret reporter; magenta - Cartpt (assay MGI:7524011).

Structure	Level	Pattern	Image	Note
TS22: hindgut	Present		3F CFP/CFP CFP , 3F CFP/CFP (merged)

3G CFP/+ (merged) ©

3G CFP/+ CFP ©

Specimen 3G CFP/+ CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret⁺ (more

)
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).

Structure	Level	Pattern	Image	Note
TS20: stomach	Present		3G CFP/+ CFP , 3G CFP/+ (merged)

3G CFP/CFP (merged) ©

3G CFP/CFP CFP ©

Specimen 3G CFP/CFP CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret^tm2.1Heno (more

)
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).

Structure	Level	Pattern	Image	Note
TS20: stomach	Present		3G CFP/CFP (merged) , 3G CFP/CFP CFP	Co-expression with GFRA2 restricted to cells along extrinsically innervating nerve fibers

3H CFP/+ (merged) ©

3H CFP/+ CFP ©

Specimen 3H CFP/+ CFP: embryonic day 14.5; Ret^tm2.1Heno/Ret⁺ (more

)
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).

Structure	Level	Pattern	Image	Note
TS22: hindgut	Present	Regionally restricted	3H CFP/+ CFP , 3H CFP/+ (merged)	Co-expression with GFRA2 in branch B matruring neurons throughout intestine.

3H CFP/CFP (merged) ©

3H CFP/CFP CFP ©

Specimen 3H CFP/CFP CFP: embryonic day 14.5; Ret^tm2.1Heno/Ret^tm2.1Heno (more

)
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).

Structure	Level	Pattern	Image	Note
TS22: hindgut	Present	Regionally restricted	3H CFP/CFP (merged) , 3H CFP/CFP CFP	Co-expressing cells restricted to exrinsically innervating nerve fibers.

5E CFP/+ (merged) ©

5E CFP/+ CFP ©

Specimen 5E CFP/+ CFP: embryonic day 14.5; Ret^tm2.1Heno/Ret⁺ (more

)
Note: Double labeled: green - Ret; magenta - phospho-histone H3.

Structure	Level	Pattern	Image	Note
TS22: stomach	Present		5E CFP/+ CFP , 5E CFP/+ (merged)

Specimen 5E CFP/CFP CFP: embryonic day 14.5; Ret^tm2.1Heno/Ret^tm2.1Heno (more

)
Note: Double labeled: green - Ret; magenta - phospho-histone H3.

Structure	Level	Pattern	Image	Note
TS22: stomach	Weak		5E CFP/CFP (merged) , 5E CFP/CFP CFP	Significant reduction in number of expressing cells compared to control.

S7B CFP/+ ©

Specimen S7B CFP/+ CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret⁺ (more

)
Note: Triple labeled: blue - Ret reporter; red - Cartpt (assay MGI:7524013); green - Gal (assay MGI:7524014).

Structure	Level	Pattern	Image	Note
TS20: stomach	Present		S7B CFP/+

S7B CFP/CFP ©

Specimen S7B CFP/CFP CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret^tm2.1Heno (more

)
Note: Triple labeled: blue - Ret reporter; red - Cartpt (assay MGI:7524013); green - Gal (assay MGI:7524014).

Structure	Level	Pattern	Image	Note
TS20: stomach	Ambiguous		S7B CFP/CFP	Expression not specifically described.

Specimen S7C CFP/+ CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret⁺ (more

)
Note: Triple labeled: blue - Ret reporter; red - Cartpt (assay MGI:7524013); green - Gal (assay MGI:7524014).

Structure	Level	Pattern	Image	Note
TS20: stomach	Present		S7C CFP/+ CFP , S7C CFP/+ (merged)

Specimen S7C CFP/CFP CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret^tm2.1Heno (more

)
Note: Triple labeled: blue - Ret reporter; red - Cartpt (assay MGI:7524013); green - Gal (assay MGI:7524014).

Structure	Level	Pattern	Image	Note
TS20: stomach	Ambiguous		S7C CFP/CFP (merged) , S7C CFP/CFP CFP	Expression not specifically described.

Specimen S7D CFP/+ CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret⁺ (more

)
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: esophagus	migratory enteric neural crest cell	Present	Regionally restricted	S7D CFP/+ CFP , S7D CFP/+ (merged)	Co-expression with GFRA2 in putative branch B cells in both genotypes. Expression in non-neuronal enteric neural crest-derived cells.

Specimen S7D CFP/CFP CFP: embryonic day 12.5; Ret^tm2.1Heno/Ret⁺ (more

)
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: esophagus	migratory enteric neural crest cell	Present	Regionally restricted	S7D CFP/CFP (merged) , S7D CFP/CFP CFP	Co-expression with GFRA2 in putative branch B cells in both genotypes. Expression in non-neuronal enteric neural crest-derived cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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