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Gene Expression Data
Assay Details
Assay
Reference: J:339398 Vincent E, et al., Ret deficiency decreases neural crest progenitor proliferation and restricts fate potential during enteric nervous system development. Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2211986120
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7523998
Gene symbol: Ret
Gene name: ret proto-oncogene
Reporter: CFP
Probe: CFP probe (529381)
Probe preparation: labelled with beta-galactosidase
Visualized with: Fluorescence
Results
Specimen 2E E12.5 CFP/+: embryonic day 12.5; Rettm2.1Heno/Ret+ (more )
Structure Cell Type Level Pattern Image Note
TS20: stomach migratory enteric neural crest cell Present Regionally restricted 2E E12.5 CFP/+ Expressing cells have formed a dense network throughout the stomach and all but end of hindgut by E12.5.
Specimen 2E E12.5 CFP/CFP: embryonic day 12.5; Rettm2.1Heno/Rettm2.1Heno (more )
Structure Cell Type Level Pattern Image Note
TS20: stomach migratory enteric neural crest cell Present Regionally restricted 2E E12.5 CFP/CFP Enteric neural crest-derived cells have not left the extrinsically innervating nerve fibers of stomach.
Specimen 2E E14.5 CFP/+ (left): embryonic day 14.5; Rettm2.1Heno/Ret+ (more )
Structure Cell Type Level Pattern Image Note
TS22: stomach migratory enteric neural crest cell Present 2E E14.5 CFP/+ (left)
Specimen 2E E14.5 CFP/CFP (left): embryonic day 14.5; Rettm2.1Heno/Rettm2.1Heno (more )
Structure Cell Type Level Pattern Image Note
TS22: stomach migratory enteric neural crest cell Present Regionally restricted 2E E14.5 CFP/CFP (left) Enteric neural crest-derived cells have not left the extrinsically innervating nerve fibers of stomach.
Specimen 2E E14.5 CFP/+ (right): embryonic day 14.5; Rettm2.1Heno/Ret+ (more )
Structure Cell Type Level Pattern Image Note
TS22: hindgut migratory enteric neural crest cell Present 2E E14.5 CFP/+ (right)
Specimen 2E E14.5 CFP/CFP (right): embryonic day 14.5; Rettm2.1Heno/Rettm2.1Heno (more )
Structure Level Pattern Image Note
TS22: hindgut Ambiguous Regionally restricted 2E E14.5 CFP/CFP (right) Enteric neural crest-derived cells have not left the extrinsically innervating nerve fibers of stomach.
Specimen 3E CFP/+ CFP: embryonic day 12.5; Rettm2.1Heno/Ret+ (more )
Note: Double labeled: green - Ret reporter; magenta - Cartpt (assay MGI:7524011).
Structure Level Pattern Image Note
TS20: stomach Present 3E CFP/+ (merged) , 3E CFP/+ CFP
Specimen 3E CFP/CFP CFP: embryonic day 12.5; Rettm2.1Heno/Rettm2.1Heno (more )
Note: Double labeled: green - Ret reporter; magenta - Cartpt (assay MGI:7524011).
Structure Level Pattern Image Note
TS20: stomach Present 3E CFP/CFP CFP , 3E CFP/CFP (merged)
Specimen 3F CFP/+ CFP: embryonic day 14.5; Rettm2.1Heno/Ret+ (more )
Note: Double labeled: green - Ret reporter; magenta - Cartpt (assay MGI:7524011).
Structure Level Pattern Image Note
TS22: hindgut Present 3F CFP/+ (merged) , 3F CFP/+ CFP
Specimen 3F CFP/CFP CFP: embryonic day 14.5; Rettm2.1Heno/Rettm2.1Heno (more )
Note: Double labeled: green - Ret reporter; magenta - Cartpt (assay MGI:7524011).
Structure Level Pattern Image Note
TS22: hindgut Present 3F CFP/CFP CFP , 3F CFP/CFP (merged)
Specimen 3G CFP/+ CFP: embryonic day 12.5; Rettm2.1Heno/Ret+ (more )
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).
Structure Level Pattern Image Note
TS20: stomach Present 3G CFP/+ CFP , 3G CFP/+ (merged)
Specimen 3G CFP/CFP CFP: embryonic day 12.5; Rettm2.1Heno/Rettm2.1Heno (more )
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).
Structure Level Pattern Image Note
TS20: stomach Present 3G CFP/CFP (merged) , 3G CFP/CFP CFP Co-expression with GFRA2 restricted to cells along extrinsically innervating nerve fibers
Specimen 3H CFP/+ CFP: embryonic day 14.5; Rettm2.1Heno/Ret+ (more )
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).
Structure Level Pattern Image Note
TS22: hindgut Present Regionally restricted 3H CFP/+ CFP , 3H CFP/+ (merged) Co-expression with GFRA2 in branch B matruring neurons throughout intestine.
Specimen 3H CFP/CFP CFP: embryonic day 14.5; Rettm2.1Heno/Rettm2.1Heno (more )
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).
Structure Level Pattern Image Note
TS22: hindgut Present Regionally restricted 3H CFP/CFP (merged) , 3H CFP/CFP CFP Co-expressing cells restricted to exrinsically innervating nerve fibers.
Specimen 5E CFP/+ CFP: embryonic day 14.5; Rettm2.1Heno/Ret+ (more )
Note: Double labeled: green - Ret; magenta - phospho-histone H3.
Structure Level Pattern Image Note
TS22: stomach Present 5E CFP/+ CFP , 5E CFP/+ (merged)
Specimen 5E CFP/CFP CFP: embryonic day 14.5; Rettm2.1Heno/Rettm2.1Heno (more )
Note: Double labeled: green - Ret; magenta - phospho-histone H3.
Structure Level Pattern Image Note
TS22: stomach Weak 5E CFP/CFP (merged) , 5E CFP/CFP CFP Significant reduction in number of expressing cells compared to control.
Specimen S7B CFP/+ CFP: embryonic day 12.5; Rettm2.1Heno/Ret+ (more )
Note: Triple labeled: blue - Ret reporter; red - Cartpt (assay MGI:7524013); green - Gal (assay MGI:7524014).
Structure Level Pattern Image Note
TS20: stomach Present S7B CFP/+
Specimen S7B CFP/CFP CFP: embryonic day 12.5; Rettm2.1Heno/Rettm2.1Heno (more )
Note: Triple labeled: blue - Ret reporter; red - Cartpt (assay MGI:7524013); green - Gal (assay MGI:7524014).
Structure Level Pattern Image Note
TS20: stomach Ambiguous S7B CFP/CFP Expression not specifically described.
Specimen S7C CFP/+ CFP: embryonic day 12.5; Rettm2.1Heno/Ret+ (more )
Note: Triple labeled: blue - Ret reporter; red - Cartpt (assay MGI:7524013); green - Gal (assay MGI:7524014).
Structure Level Pattern Image Note
TS20: stomach Present S7C CFP/+ CFP , S7C CFP/+ (merged)
Specimen S7C CFP/CFP CFP: embryonic day 12.5; Rettm2.1Heno/Rettm2.1Heno (more )
Note: Triple labeled: blue - Ret reporter; red - Cartpt (assay MGI:7524013); green - Gal (assay MGI:7524014).
Structure Level Pattern Image Note
TS20: stomach Ambiguous S7C CFP/CFP (merged) , S7C CFP/CFP CFP Expression not specifically described.
Specimen S7D CFP/+ CFP: embryonic day 12.5; Rettm2.1Heno/Ret+ (more )
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).
Structure Cell Type Level Pattern Image Note
TS20: esophagus migratory enteric neural crest cell Present Regionally restricted S7D CFP/+ CFP , S7D CFP/+ (merged) Co-expression with GFRA2 in putative branch B cells in both genotypes. Expression in non-neuronal enteric neural crest-derived cells.
Specimen S7D CFP/CFP CFP: embryonic day 12.5; Rettm2.1Heno/Ret+ (more )
Note: Double labeled: green - Ret reporter; magenta - Gfra2 (assay MGI:7524012).
Structure Cell Type Level Pattern Image Note
TS20: esophagus migratory enteric neural crest cell Present Regionally restricted S7D CFP/CFP (merged) , S7D CFP/CFP CFP Co-expression with GFRA2 in putative branch B cells in both genotypes. Expression in non-neuronal enteric neural crest-derived cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory