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Gene Expression Data
Assay Details
Assay
Reference: J:65135 Schwarting GA, et al., Semaphorin 3A is required for guidance of olfactory axons in mice. J Neurosci. 2000 Oct 15;20(20):7691-7
Assay type: RNA in situ
MGI Accession ID: MGI:7522012
Gene symbol: Sema3a
Gene name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Probe: SemD cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1A: embryonic day 13.5 (more )
Note: Rostral olfactory bulb.
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted 1A Expression is in a sheet of cells in the nerve layer surrounding the olfactory bulb.
TS21: main olfactory bulb layer Present Regionally restricted 1A Expression is in the nerve layer medially and ventrally in the rostral olfactory bulb.
TS21: head mesenchyme Present 1A
Specimen 1C: embryonic day 13.5 (more )
Note: Medial olfactory bulb.
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted 1C Expression is in a sheet of cells in the nerve layer surrounding the olfactory bulb.
TS21: main olfactory bulb layer Present Regionally restricted 1C Expression is in the nerve layer medially and ventrally in the central olfactory bulb.
TS21: head mesenchyme Present 1C
Specimen 1E: embryonic day 13.5 (more )
Note: Caudal olfactory bulb.
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted 1E , 1G Expression is in a sheet of cells in the nerve layer surrounding the olfactory bulb.
TS21: main olfactory bulb layer Present Regionally restricted 1E , 1G In the caudal olfactory bulb expression in the ventral nerve layer has gaps in expression.
Specimen 2A: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: main olfactory bulb olfactory nerve layer Present Regionally restricted 2A Expression is in the outer nerve layer in a block of cells distributed along the ventral surface of the olfactory bulb, with small gaps near the rostral and caudal ends.
Specimen 2C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: main olfactory bulb olfactory nerve layer Present Regionally restricted 2C There large gaps in expression in the medial and ventral nerve layer that flank a region of heavy expression in the ventromedial nerve layer.
Specimen not shown E12: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: olfactory epithelium Present Regionally restricted Expression is in non-neuronal cells along axon pathways between the olfactrory epithelium and the olfactory bulb.
TS20: telencephalon Present Regionally restricted Expression is in non-neuronal cells along axon pathways between the olfactrory epithelium and the olfactory bulb.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory